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Reply to “Uncoupling diffusion and binding in FRAP experiments”

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Figure 1: Spatial profile of the first Lck-CFP-YFP molecules entering the bleached spot.

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Acknowledgements

This work was supported by the Austrian Science Fund (project Y250-B10), the Competence Center for Biomolecular Therapeutics Research–Vienna and by the GEN-AU project of the Austrian Federal Ministry for Science and Research.

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Authors and Affiliations

  1. Biophysics Institute, Johannes Kepler University Linz, Altenbergerstr. 69, Linz, A-4040, Austria

    Mario Brameshuber, Michaela Schwarzenbacher, Martin Kaltenbrunner, Clemens Hesch, Julian Weghuber & Gerhard J Schütz

  2. Department of Molecular Immunology, Center for Physiology, Pathophysiology and Immunology, Medical University of Vienna, Lazarettgasse 19, Vienna, A-1090, Austria

    Wolfgang Paster & Hannes Stockinger

  3. Department of Knowledge-based Mathematical Systems, Johannes Kepler University Linz, Altenbergerstr. 69, Linz, A-4040, Austria

    Bettina Heise

  4. Center for Biomedical Nanotechnology, Upper Austrian Research GmbH, Scharitzerstr. 6-8, Linz, A-4020, Austria

    Alois Sonnleitner

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  1. Mario Brameshuber
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  2. Michaela Schwarzenbacher
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  9. Hannes Stockinger
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  10. Gerhard J Schütz
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Corresponding authors

Correspondence to Hannes Stockinger or Gerhard J Schütz.

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Brameshuber, M., Schwarzenbacher, M., Kaltenbrunner, M. et al. Reply to “Uncoupling diffusion and binding in FRAP experiments”. Nat Methods 6, 183–184 (2009). https://doi.org/10.1038/nmeth0309-183b

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