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A red-shifted Renilla luciferase for transient reporter-gene expression

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Figure 1: RLuc7-521 experimental validation.

References

  1. Zhao, H. et al. J. Biomed. Opt. 10, 41210 (2005).

    Article  Google Scholar 

  2. Loening, A.M., Wu, A.M. & Gambhir, S.S. Nat. Methods 4, 641–643 (2007).

    Article  CAS  Google Scholar 

  3. Loening, A.M., Fenn, T.D., Wu, A.M. & Gambhir, S.S. Protein Eng. Des. Sel. 19, 391–400 (2006).

    Article  CAS  Google Scholar 

  4. Loening, A.M., Fenn, T.D. & Gambhir, S.S. J. Mol. Biol. 374, 1017–1028 (2007).

    Article  CAS  Google Scholar 

  5. Woo, J., Howell, M. & von Arnim, A. Protein Sci. 17, 725–735 (2008).

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by the Stanford Medical Scientist Training Program (A.M.L.), US National Cancer Institute (NCI) CA114747 In Vivo Cellular and Molecular Imaging Center P50 (S.S.G.), NCI CA119367 Centers of Cancer Nanotechnology Excellence U54 (S.S.G.), NCI R01 CA082214 (S.S.G.) and the NCI Small Animal Imaging Resource Program. The authors thank B. Rice and T. Troy of Caliper Life Sciences for help with spectral measurements and Z. Walls and J. Park for experimental assistance.

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Authors and Affiliations

  1. Departments of Radiology and Bioengineering, Molecular Imaging Program at Stanford and Bio-X Program, Stanford University, Stanford, California

    Andreas Markus Loening, Anca Dragulescu-Andrasi & Sanjiv Sam Gambhir

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  1. Andreas Markus Loening
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  2. Anca Dragulescu-Andrasi
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  3. Sanjiv Sam Gambhir
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Correspondence to Sanjiv Sam Gambhir.

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Stanford University, where the authors are employed, has filed a patent application that covers portions of this work.

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Supplementary Figures 1 and 2, Supplementary Table 1, Supplementary Results and Supplementary Methods (PDF 285 kb)

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Loening, A., Dragulescu-Andrasi, A. & Gambhir, S. A red-shifted Renilla luciferase for transient reporter-gene expression. Nat Methods 7, 5–6 (2010). https://doi.org/10.1038/nmeth0110-05

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