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CRISPR-Cas9-AID base editor is a powerful gain-of-function screening tool

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Combining CRISPR-Cas9 with an enzyme that induces somatic hypermutations allows the rapid generation of diverse variants for gain-of-function screens.

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Figure 1: Schematics of CRISPR-AID-mediated somatic hypermutations.

References

  1. Ma, Y. et al. Nat. Methods 13, 1029–1035 (2016).

    Article  CAS  Google Scholar 

  2. Hess, G. et al. Nat. Methods 13, 1036–1042 (2016).

    Article  CAS  Google Scholar 

  3. Horvath, P. & Barrangou, R. Science 327, 167–170 (2010).

    Article  CAS  Google Scholar 

  4. Jinek, M. et al. Science 337, 816–821 (2012).

    Article  CAS  Google Scholar 

  5. Jinek, M. et al. eLife 2, e00471 (2013).

    Article  Google Scholar 

  6. Mali, P. et al. Science 339, 823–826 (2013).

    Article  CAS  Google Scholar 

  7. Cong, L. et al. Science 339, 819–823 (2013).

    Article  CAS  Google Scholar 

  8. Komor, A.C., Kim, Y.B., Packer, M.S., Zuris, J.A. & Liu, D.R. Nature 533, 420–424 (2016).

    Article  CAS  Google Scholar 

  9. Nishida, K. et al. Science http://dx.doi.org/10.1126/science.aaf8729 (2016).

  10. Odegard, V.H. & Schatz, D.G. Nat. Rev. Immunol. 6, 573–583 (2006).

    Article  CAS  Google Scholar 

  11. Findlay, G.M., Boyle, E.A., Hause, R.J., Klein, J.C. & Shendure, J. Nature 513, 120–123 (2014).

    Article  CAS  Google Scholar 

  12. Ryan, O.W. et al. eLife http://dx.doi.org/10.7554/eLife.03703 (2014).

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Authors and Affiliations

  1. Cem Kuscu and Mazhar Adli are in the Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, Virginia, USA.,

    Cem Kuscu & Mazhar Adli

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  1. Cem Kuscu
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  2. Mazhar Adli
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Correspondence to Mazhar Adli.

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Kuscu, C., Adli, M. CRISPR-Cas9-AID base editor is a powerful gain-of-function screening tool. Nat Methods 13, 983–984 (2016). https://doi.org/10.1038/nmeth.4076

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