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Removing roadblocks to deep sequencing of modified RNAs

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By removing modified nucleotides that block reverse transcriptase, two methods have now made tRNAs amenable to RNA-seq.

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Figure 1: Removing methylated nucleotides to sequence tRNAs.

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Acknowledgements

J.E.W. is supported by US National Institutes of Health grant R00-GM104166 and the Rita Allen Foundation.

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  1. Jeremy E. Wilusz is in the Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania, USA.,

    Jeremy E Wilusz

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  1. Jeremy E Wilusz
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Correspondence to Jeremy E Wilusz.

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Wilusz, J. Removing roadblocks to deep sequencing of modified RNAs. Nat Methods 12, 821–822 (2015). https://doi.org/10.1038/nmeth.3516

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