In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.
References
Cox, I.J. J. Microsc. 133, 149–154 (1984).
Wijnaendts Van Resandt, R.W. et al. J. Microsc. 138, 29–34 (1985).
Wagner, M. et al. Int. J. Mol. Sci. 11, 956–966 (2010).
Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H.K. Science 305, 1007–1009 (2004).
Greger, K., Swoger, J. & Stelzer, E.H.K. Rev. Sci. Instrum. 78, 023705 (2007).
Keller, P.J., Schmidt, A.D., Wittbrodt, J. & Stelzer, E.H.K. Science 322, 1065–1069 (2008).
Wilson, T. & Sheppard, C.J.R. Theory and Practice of Scanning Optical Microscopy (Academic Press, 1984).
Breuninger, T., Greger, K. & Stelzer, E.H.K. Opt. Lett. 32, 1938–1940 (2007).
Keller, P.J. et al. Nat. Methods 7, 637–642 (2010).
Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M. & Fraser, S.E. Nat. Methods 8, 757–760 (2011).
Fahrbach, F.O. & Rohrbach, A. Opt. Express 18, 24229–24244 (2010).
Vettenburg, T. et al. Nat. Methods 11, 541–544 (2014).
Cella Zanacchi, F. et al. Nat. Methods 8, 1047–1049 (2011).
Huang, B., Wang, W., Bates, M. & Zhuang, X. Science 319, 810–813 (2008).
Hell, S.W. & Wichmann, J. Opt. Lett. 19, 780–782 (1994).
Chen, B.-C. et al. Science 346, 1257998 (2014).
Gustafsson, M.G.L. J. Microsc. 198, 82–87 (2000).
Siedentopf, H. & Zsigmondy, R. Ann. Phys. 315, 1–39 (1903).
Voie, A.H., Burns, D.H. & Spelman, F.A. J. Microsc. 170, 229–236 (1993).
Verveer, P.J. et al. Nat. Methods 4, 311–313 (2007).
Swoger, J., Verveer, P., Greger, K., Huisken, J. & Stelzer, E.H.K. Opt. Express 15, 8029–8042 (2007).
Hell, S. & Stelzer, E.H.K. J. Opt. Soc. Am. A 9, 2159–2166 (1992).
Stelzer, E.H.K. & Lindek, S. Opt. Commun. 111, 536–547 (1994).
Strobl, F. & Stelzer, E.H.K. Development 141, 2331–2338 (2014).
Maizel, A., von Wangenheim, D., Federici, F., Haseloff, J. & Stelzer, E.H.K. Plant J. 68, 377–385 (2011).
Wohland, T., Shi, X.K., Sankaran, J. & Stelzer, E.H.K. Opt. Express 18, 10627–10641 (2010).
Greger, K., Neetz, M.J., Reynaud, E.G. & Stelzer, E.H.K. Opt. Express 19, 20743–20750 (2011).
Preibisch, S. et al. Nat. Methods 11, 645–648 (2014).
Wu, Y. et al. Proc. Natl. Acad. Sci. USA 108, 17708–17713 (2011).
Pampaloni, F. et al. Integr. Biol. (Camb.) 6, 988–998 (2014).
Acknowledgements
E.H.K.S.'s research is funded through CEF-MC (EXC 115) by the Deutsche Forschungsgemeinschaft.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
E.H.K.S. is an inventor or co-inventor of two relevant patents owned by the European Molecular Biology Laboratory Enterprise Management Technology Transfer GmbH: (i) microscope with a viewing direction perpendicular to the illumination direction, US 7554725; DE 10257423 and (ii) single plane illumination microscope US 20070109633 A1; PCT/EP03/05991.
Rights and permissions
About this article
Cite this article
Stelzer, E. Light-sheet fluorescence microscopy for quantitative biology. Nat Methods 12, 23–26 (2015). https://doi.org/10.1038/nmeth.3219
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.3219
- Springer Nature America, Inc.
This article is cited by
-
Current and future applications of light-sheet imaging for identifying molecular and developmental processes in autism spectrum disorders
Molecular Psychiatry (2024)
-
Vascularized organoid-on-a-chip: design, imaging, and analysis
Angiogenesis (2024)
-
Acoustic prison for single live cell 3D multi-imaging enabled by light-sheet microscopy
Microfluidics and Nanofluidics (2023)
-
Identification of a Suitable Untargeted Agent for the Clinical Translation of ABY-029 Paired-Agent Imaging in Fluorescence-Guided Surgery
Molecular Imaging and Biology (2023)
-
Structural and functional imaging of brains
Science China Chemistry (2023)