The idea of using hybrid proteins containing transcription factor domains to analyze protein-protein interactions was described in 1989. Over the past 25 years, this method has begun to reveal the complex protein networks that underlie cellular behavior.
References
Fields, S. & Song, O. Nature 340, 245–246 (1989).
Chien, C.T., Bartel, P.L., Sternglanz, R. & Fields, S. Proc. Natl. Acad. Sci. USA 88, 9578–9582 (1991).
Vidal, M. & Legrain, P. Nucleic Acids Res. 27, 919–929 (1999).
Li, J.J. & Herskowitz, I. Science 262, 1870–1874 (1993).
Wang, M.M. & Reed, R.R. Nature 364, 121–126 (1993).
SenGupta, D.J. et al. Proc. Natl. Acad. Sci. USA 93, 8496–8501 (1996).
Licitra, E.J. & Liu, J.O. Proc. Natl. Acad. Sci. USA 93, 12817–12821 (1996).
Johnsson, N. & Varshavsky, A. Proc. Natl. Acad. Sci. USA 91, 10340–10344 (1994).
Pelletier, J.N., Campbell-Valois, F.X. & Michnick, S.W. Proc. Natl. Acad. Sci. USA 95, 12141–12146 (1998).
Eyckerman, S. et al. Nat. Cell Biol. 3, 1114–1119 (2001).
Ramachandran, N. et al. Science 305, 86–90 (2004).
Vidal, M., Brachmann, R.K., Fattaey, A., Harlow, E. & Boeke, J.D. Proc. Natl. Acad. Sci. USA 93, 10315–10320 (1996).
Dreze, M. et al. Nat. Methods 6, 843–849 (2009).
Oliver, S.G. et al. Nature 357, 38–46 (1992).
Sulston, J. et al. Nature 356, 37–41 (1992).
Bartel, P.L., Roecklein, J.A., SenGupta, D. & Fields, S. Nat. Genet. 12, 72–77 (1996).
Fromont-Racine, M., Rain, J.C. & Legrain, P. Nat. Genet. 16, 277–282 (1997).
Walhout, A.J. & Vidal, M. Genome Res. 9, 1128–1134 (1999).
Uetz, P. et al. Nature 403, 623–627 (2000).
Ito, T. et al. Proc. Natl. Acad. Sci. USA 98, 4569–4574 (2001).
Walhout, A.J. et al. Science 287, 116–122 (2000).
Li, S. et al. Science 303, 540–543 (2004).
Giot, L. et al. Science 302, 1727–1736 (2003).
Rual, J.-F. et al. Nature 437, 1173–1178 (2005).
Stelzl, U. et al. Cell 122, 957–968 (2005).
Gavin, A.C. et al. Nature 415, 141–147 (2002).
Gavin, A.C. et al. Nature 440, 631–636 (2006).
Ho, Y. et al. Nature 415, 180–183 (2002).
Krogan, N.J. et al. Nature 440, 637–643 (2006).
Rolland, T. et al. Cell (in the press).
Venkatesan, K. et al. Nat. Methods 6, 83–90 (2009).
Ge, H., Walhout, A.J. & Vidal, M. Trends Genet. 19, 551–560 (2003).
Han, J.D. et al. Nature 430, 88–93 (2004).
Gu, L. et al. Nature doi:10.1038/nature13761 (21 September 2014).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Vidal, M., Fields, S. The yeast two-hybrid assay: still finding connections after 25 years. Nat Methods 11, 1203–1206 (2014). https://doi.org/10.1038/nmeth.3182
Published:
Issue Date:
DOI: https://doi.org/10.1038/nmeth.3182
- Springer Nature America, Inc.
This article is cited by
-
Increased talin–vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections
Laboratory Investigation (2020)
-
Maximizing binary interactome mapping with a minimal number of assays
Nature Communications (2019)
-
Human enterovirus 71 protein interaction network prompts antiviral drug repositioning
Scientific Reports (2017)
-
Coevolutionary analyses require phylogenetically deep alignments and better null models to accurately detect inter-protein contacts within and between species
BMC Bioinformatics (2015)