Skip to main content
SpringerLink
Log in

E-CRISP: fast CRISPR target site identification

  • Correspondence
  • Published:

From Nature Methods

View current issue Submit your manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: E-CRISP workflow and example.

References

  1. Mali, P., Yang, L., Esvelt, K., Aach, J. & Guell, M. Science 339, 823–826 (2013).

    Article  CAS  Google Scholar 

  2. Cong, L., Ran, F., Cox, D., Lin, S. & Barretto, R. Science 339, 819–823 (2013).

    Article  CAS  Google Scholar 

  3. Cho, S.W., Kim, S., Kim, J. & Kim, J. Nat. Biotechnol. 31, 230–232 (2013).

    Article  CAS  Google Scholar 

  4. Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. Nat. Biotechnol. 31, 233–239 (2013).

    Article  CAS  Google Scholar 

  5. Jinek, M. et al. Elife 2, e00471 (2013).

    Article  Google Scholar 

  6. Hwang, W.Y. et al. Nat. Biotechnol. 31, 227–229 (2013).

    Article  CAS  Google Scholar 

  7. Barrangou, R., Fremaux, C. & Deveau, H. Science 315, 1709–1712 (2007).

    Article  CAS  Google Scholar 

  8. Horvath, P. & Barrangou, R. Science 327, 167–170 (2010).

    Article  CAS  Google Scholar 

  9. Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V. Proc. Natl. Acad. Sci. USA 109, E2579–E2586 (2012).

    Article  CAS  Google Scholar 

  10. Deltcheva, E. et al. Nature 471, 602–607 (2011).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank M. Breinig, N. Walther, V. Chaudhary, K. Gläser, O. Pelz and other members of the Boutros laboratory for discussions. Research in the group of M.B. is supported by a European Research Council Advanced Grant of the European Commission.

Author information

Authors and Affiliations

  1. Division of Signaling and Functional Genomics, German Cancer Research Center (DKFZ), Heidelberg, Germany

    Florian Heigwer, Grainne Kerr & Michael Boutros

  2. Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany

    Florian Heigwer, Grainne Kerr & Michael Boutros

Authors
  1. Florian Heigwer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Grainne Kerr
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael Boutros
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Michael Boutros.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Figure, Table and Notes

Supplementary Figure 1, Supplementary Table 1 and Supplementary Notes 1 and 2 (PDF 144 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Heigwer, F., Kerr, G. & Boutros, M. E-CRISP: fast CRISPR target site identification. Nat Methods 11, 122–123 (2014). https://doi.org/10.1038/nmeth.2812

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.2812

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation