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Deciphering laminar-specific neural inputs with line-scanning fMRI

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Abstract

Using a line-scanning method during functional magnetic resonance imaging (fMRI), we obtained high temporal (50-ms) and spatial (50-μm) resolution information along the cortical thickness and showed that the laminar position of fMRI onset coincides with distinct neural inputs in rat somatosensory and motor cortices. This laminar-specific fMRI onset allowed us to identify the neural inputs underlying ipsilateral fMRI activation in the barrel cortex due to peripheral denervation-induced plasticity.

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Figure 1: Characterization of the line-scanning fMRI method in rat somatosensory and motor cortex.
Figure 2: Mapping the inputs into the barrel cortex (BC) in a rat model of cortical plasticity.

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Acknowledgements

This research was supported by the Intramural Research Program of the US National Institutes of Health–NINDS. We thank K. Sharer and N. Bouraoud for technical support.

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Authors and Affiliations

  1. Laboratory of Functional and Molecular Imaging, National Institute of Neurological Disorders and Stroke, US National Institutes of Health, Bethesda, Maryland, USA

    Xin Yu, Chunqi Qian, Der-yow Chen, Stephen J Dodd & Alan P Koretsky

Authors
  1. Xin Yu
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  2. Chunqi Qian
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  3. Der-yow Chen
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  4. Stephen J Dodd
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  5. Alan P Koretsky
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Contributions

X.Y. and A.P.K. conceived of the line-scanning strategy and designed experiments. X.Y. established the line-scanning method, performed experiments and analyzed the data. C.Q. and D.-y.C. performed blind experiments on the MEMRI tracing and line-scanning fMRI of the plasticity model. S.J.D. provided magnetic resonance technical support and IDL (Interactive Data Language) analytical tools. X.Y. and A.P.K. wrote the paper.

Corresponding authors

Correspondence to Xin Yu or Alan P Koretsky.

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The authors declare no competing financial interests.

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Supplementary Figures 1–18, Supplementary Tables 1–5 and Supplementary Notes 1 and 2 (PDF 6252 kb)

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Yu, X., Qian, C., Chen, Dy. et al. Deciphering laminar-specific neural inputs with line-scanning fMRI. Nat Methods 11, 55–58 (2014). https://doi.org/10.1038/nmeth.2730

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