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FISH-quant: automatic counting of transcripts in 3D FISH images

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Figure 1: Counting mature and nascent mRNA in FISH-quant.

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Acknowledgements

This research was supported by the Agence Nationale de la Recherche (ANR-10-BLAN-1222, ANR-09-PIRI-0024, ANR-10-INTB-1401), Sidaction, the Région Ile-de-France under C'Nano IdF (the Center of Competences in NanoSciences for the Paris region), the Fondation pour la Recherche Médicale en France (FRM) and the Institut Pasteur. We thank J. McNally for editing the manuscript.

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Authors and Affiliations

  1. Institut Pasteur, Unité Imagerie et Modélisation, Centre National de la Recherche Scientifique (CNRS) Unité de Recherche Associée 2582, Paris, France

    Florian Mueller, Hervé Marie-Nelly & Christophe Zimmer

  2. Imagerie de la machinerie transcriptionnelle, Institut de Biologie de l'Ecole Normale Supérieure, CNRS Unité Mixte de Recherche 8197, Paris, France

    Florian Mueller, Adrien Senecal, Nathalie Ly, Olivier Collin & Xavier Darzacq

  3. Institut de Génétique Moléculaire de Montpellier, CNRS, Unité Mixte de Recherche 5535, Montpellier, France

    Katjana Tantale, Eugenia Basyuk & Edouard Bertrand

Authors
  1. Florian Mueller
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  2. Adrien Senecal
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  3. Katjana Tantale
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  4. Hervé Marie-Nelly
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  5. Nathalie Ly
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  6. Olivier Collin
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  7. Eugenia Basyuk
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  8. Edouard Bertrand
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  9. Xavier Darzacq
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  10. Christophe Zimmer
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Corresponding authors

Correspondence to Edouard Bertrand, Xavier Darzacq or Christophe Zimmer.

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The authors declare no competing financial interests.

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Supplementary Notes 1–6 and Supplementary Methods (PDF 8880 kb)

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Mueller, F., Senecal, A., Tantale, K. et al. FISH-quant: automatic counting of transcripts in 3D FISH images. Nat Methods 10, 277–278 (2013). https://doi.org/10.1038/nmeth.2406

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