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Fluorescence and super-resolution standards based on DNA origami

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Figure 1: DNA origami standards for fluorescence microscopy.

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Acknowledgements

We thank V. Schüller, R. Schreiber, E. Pibiri, I. Burkhardt, R. Jungmann, P. Nickels, T. Liedl, B. Lalkens and D. Grohmann for help with experiments and fruitful discussions. We are grateful to W. Fouquet, J. Sieber and Leica Microsystems for STED measurements. Financial support by the Biophotonics IV program of the Federal Ministry of Education and Research (BMBF)/Association of German Engineers (VDI)(13N11461) and the German Research Foundation (DFG Ti329/6-1) is gratefully acknowledged.

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Author notes

  1. Jürgen J Schmied, Andreas Gietl and Phil Holzmeister: These authors contributed equally to this work.

Authors and Affiliations

  1. Institute for Physical and Theoretical Chemistry, Braunschweig University of Technology, Braunschweig, Germany

    Jürgen J Schmied, Andreas Gietl, Phil Holzmeister, Carsten Forthmann, Thorben Dammeyer & Philip Tinnefeld

  2. STS Nanotechnology UG, Freising, Germany

    Christian Steinhauer

Authors
  1. Jürgen J Schmied
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  2. Andreas Gietl
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  3. Phil Holzmeister
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  4. Carsten Forthmann
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  5. Christian Steinhauer
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  6. Thorben Dammeyer
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  7. Philip Tinnefeld
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Corresponding author

Correspondence to Philip Tinnefeld.

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Competing interests

Braunschweig University of Technology, which J.J.S., A.G., P.H., C.F., T.D. and P.T. are affiliated with, has filed a provisional patent application for DNA origami–based standards.

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Supplementary Figures 1–8 and Supplementary Methods (PDF 7992 kb)

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Schmied, J., Gietl, A., Holzmeister, P. et al. Fluorescence and super-resolution standards based on DNA origami. Nat Methods 9, 1133–1134 (2012). https://doi.org/10.1038/nmeth.2254

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