A new high-throughput method for monitoring G protein–coupled receptor activation is highly suited to assaying Gα12/13-coupled receptors and is used to deorphanize a group of receptors activated by lysophosphatidylserine.
References
Inoue, A. et al. Nat. Methods 9, 1021–1029 (2012).
Fredriksson, R. & Schiöth, H.B. Mol. Pharmacol. 67, 1414–1425 (2005).
Jacoby, E. et al. ChemMedChem 1, 761–782 (2006).
Thomsen, W., Frazer, J. & Unett, D. Curr. Opin. Biotechnol. 16, 655–665 (2005).
Barnea, G et al. Proc. Natl. Acad. Sci. USA 105, 64–69 (2008).
Zhang, J.H., Chung, T.D. & Oldenburg, K.R. J. Biomol. Screen. 4, 67–73 (1999).
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Parmentier, M. Shedding light on G protein–coupled receptor signaling. Nat Methods 9, 965–966 (2012). https://doi.org/10.1038/nmeth.2178
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DOI: https://doi.org/10.1038/nmeth.2178
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