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Reprogramming in suspension

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Current practice for the generation and maintenance of induced pluripotent stem cells (iPSCs) involves static culture in dishes. Two groups now report that mouse iPSCs can be generated efficiently in stirred suspension culture.

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Figure 1: Suspension-culture reprogramming.

References

  1. Fluri, D.A. et al. Nat. Methods 9, 509–516 (2012).

    Article  CAS  Google Scholar 

  2. Shafa, M. et al. Nat. Methods 9, 465–466 (2012).

    Article  CAS  Google Scholar 

  3. Maherali, N. & Hochedlinger, K. Cell Stem Cell 3, 595–605 (2008).

    Article  CAS  Google Scholar 

  4. Yamanaka, S. & Blau, H.M. Nature 465, 704–712 (2010).

    Article  CAS  Google Scholar 

  5. Zhou, T. et al. J. Am. Soc. Nephrol. 22, 1221–1228 (2011).

    Article  Google Scholar 

  6. Robinton, D.A. & Daley, G.Q. Nature 481, 295–305 (2012).

    Article  CAS  Google Scholar 

  7. Li, R. et al. Cell Stem Cell 7, 51–63 (2010).

    Article  CAS  Google Scholar 

  8. Zhu, S., Wei, W. & Ding, S. Annu. Rev. Biomed. Eng. 13, 73–90 (2011).

    Article  Google Scholar 

  9. Chen, J. et al. Cell Res. 21, 884–894 (2011).

    Article  CAS  Google Scholar 

  10. Chen, G. et al. Nat. Methods 8, 424–429 (2011).

    Article  CAS  Google Scholar 

  11. Steiner, D. et al. Nat. Biotechnol. 28, 361–364 (2010).

    Article  CAS  Google Scholar 

  12. Andang, M. et al. Nat. Protoc. 3, 1013–1017 (2008).

    Article  Google Scholar 

  13. Pei, D. J. Biol. Chem. 284, 3365–3369 (2009).

    Article  CAS  Google Scholar 

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Correspondence to Duanqing Pei.

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Chen, J., Pei, D. Reprogramming in suspension. Nat Methods 9, 449–451 (2012). https://doi.org/10.1038/nmeth.1989

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