Skip to main content
SpringerLink
Log in

Recognizing heart cells in a crowd

  • News & Views
  • Published:

From Nature Methods

View current issue Submit your manuscript

A cardiac-specific reporter genetically engineered into human embryonic stem cells allows the optimization of differentiation protocols and the identification of cell-surface markers—a welcome new tool to help isolate and define cardiac cell lineages.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Applications of the NKX2-5eGFP/w reporter cell line.

References

  1. Elliott, D.A. et al. Nat. Methods 8, 1037–1040 (2011).

    Article  CAS  Google Scholar 

  2. Dubois, N.C. et al. Nat. Biotechnol. published online, 10.1038/nbt.2005 (23 October 2011).

  3. Wu, S.M. et al. Cell 127, 1137–1150 (2006).

    Article  CAS  Google Scholar 

  4. Christoforou, N. et al. J. Clin. Invest. 118, 894–903 (2008).

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Masino, A.M. et al. Circ. Res. 95, 389–397 (2004).

    Article  CAS  Google Scholar 

  6. Bu, L. et al. Nature 460, 113–117 (2009).

    Article  CAS  Google Scholar 

  7. Kita-Matsuo, H. et al. PLoS ONE 4, e5046 (2009).

    Article  Google Scholar 

  8. He, J.Q., Ma, Y., Lee, Y., Thomson, J.A. & Kamp, T.J. Circ. Res. 93, 32–39 (2003).

    Article  CAS  Google Scholar 

  9. Carvajal-Vergara, X. et al. Nature 465, 808–812 (2010).

    Article  CAS  Google Scholar 

  10. Moretti, A. et al. N. Engl. J. Med. 363, 1397–1409 (2010).

    Article  CAS  Google Scholar 

  11. Ieda, M. et al. Cell 142, 375–386 (2010).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medicine and Division of Cardiovascular Medicine, Timothy J. Kamp is at the University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA.,

    Timothy J Kamp

Authors
  1. Timothy J Kamp
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Timothy J Kamp.

Ethics declarations

Competing interests

T.J.K. is a co-founder and consultant for Cellular Dynamics International, a company that uses human pluripotent stem cells and their derivatives for research, drug discovery and drug testing.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kamp, T. Recognizing heart cells in a crowd. Nat Methods 8, 1013–1016 (2011). https://doi.org/10.1038/nmeth.1780

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth.1780

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation