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A bright and photostable photoconvertible fluorescent protein

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A Corrigendum to this article was published on 29 June 2011

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Abstract

Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the ∼10-nm localization precision of its parent.

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Figure 1: Tracking dynamics in live cells with mEos2 fusions.
Figure 2: Imaging of mEos2 fusions.

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Change history

  • 10 May 2011

    In the version of this supplementary file originally posted online, the mitotic phases were mislabeled in Supplementary Figure 10; during amendment of the figure legend, two of the mitotic phases (metaphase and prophase) could not be tracked back to the original data. The images have been replaced, and the labeling of mitotic phases has been corrected in this file as of 10 May 2011.

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Acknowledgements

We thank H. White and S. Winfrey for performing cell cultures and transfections, E. Betzig and H. Shroff (Janelia Farm Research Campus) for help with PALM imaging and for providing their analysis software, and E. Ramko and K. Wilson for technical assistance with fusion-vector construction.

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Authors and Affiliations

  1. Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, 20147, Virginia, USA

    Sean A McKinney & Loren L Looger

  2. National High Magnetic Field Laboratory and Department of Biological Science, The Florida State University, 1800 E. Paul Dirac Dr., Tallahassee, 32310, Florida, USA

    Christopher S Murphy, Kristin L Hazelwood & Michael W Davidson

Authors
  1. Sean A McKinney
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  2. Christopher S Murphy
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  3. Kristin L Hazelwood
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  4. Michael W Davidson
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  5. Loren L Looger
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Corresponding authors

Correspondence to Michael W Davidson or Loren L Looger.

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Supplementary Text and Figures

Supplementary Figures 1–13, Supplementary Tables 1–2, Supplementary Methods (PDF 5107 kb)

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McKinney, S., Murphy, C., Hazelwood, K. et al. A bright and photostable photoconvertible fluorescent protein. Nat Methods 6, 131–133 (2009). https://doi.org/10.1038/nmeth.1296

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  • DOI: https://doi.org/10.1038/nmeth.1296

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