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Interpreting flow cytometry data: a guide for the perplexed

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Recent advances in flow cytometry technologies are changing how researchers collect, look at and present their data.

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Figure 1: 'Logicle' displays provide improved representation of cells with minimal fluorescence.
Figure 2: 'Logicle' displays are superior to logarithmic displays in determining whether compensation has been properly applied to the sample.
Figure 3: Quantile contour plots provide more accurate data representation than dot plots.

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Authors and Affiliations

  1. Department of Genetics, Stanford University School of Medicine, Stanford, 94305-5318, California, USA

    Leonore A Herzenberg, James Tung, Wayne A Moore, Leonard A Herzenberg & David R Parks

Authors
  1. Leonore A Herzenberg
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  2. James Tung
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  3. Wayne A Moore
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  4. Leonard A Herzenberg
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  5. David R Parks
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Herzenberg, L., Tung, J., Moore, W. et al. Interpreting flow cytometry data: a guide for the perplexed. Nat Immunol 7, 681–685 (2006). https://doi.org/10.1038/ni0706-681

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