Given the right sequence of cues, embryonic stem cells can develop into any cell type found in the body. New work shows how manipulation of supportive stromal cells to provide essential Delta-Notch interactions allows embryonic stem cells to develop into functional T lymphocytes during in vitro culture.
References
Schmitt, T.M. et al. Nat. Immunol. 5, 410–417 (2004).
Olson, M.C. et al. Immunity 3, 703–714 (1995).
Simon, M.C. et al. Nat. Genet. 1, 92–98 (1992).
Nakano, T., Kodama, H. & Honjo, T. Science 265, 1098–1101 (1994).
Potocnik, A.J., Nielsen, P.J. & Eichmann, K. EMBO J. 13, 5274–5283 (1994).
De Pooter, R.F., Cho, S.K., Carlyle, J.R. & Zúñiga-Pflücker, J.C. Blood 102, 1649–1953 (2003).
Schmitt, T.M. & Zúñiga-Pflücker, J.C. Immunity 17, 749–756 (2002).
Zúñiga-Pflücker, J.C. Nat. Rev. Immunol. 4, 67–72 (2004).
Pui, J.C. et al. Immunity 11, 299–308 (1999).
Radtke, F. et al. Immunity 10, 547–558 (1999).
Jaleco, A.C. et al. J. Exp. Med. 194, 991–1002 (2001).
Fisher, R.C. et al. Mol. Cell. Biol. 18, 4347–4357 (1998).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rothenberg, E. From totipotency to T in a dish. Nat Immunol 5, 359–360 (2004). https://doi.org/10.1038/ni0404-359
Issue Date:
DOI: https://doi.org/10.1038/ni0404-359
- Springer Nature America, Inc.
This article is cited by
-
From stem cell to T cell in vitro
Nature Reviews Immunology (2004)