Abstract
Next-generation sequencing technologies need careful design of experiments and evaluation of results to meet field requirements. Here we discuss technical considerations for these high-throughput assays, together with criteria to assess the quality of the results and the necessary validation.
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Acknowledgements
The work of A.M. on this manuscript was supported by the University of California Irvine Center for Complex Biological Systems and US National Institutes of Health P50 GM076516. We thank I. Blitz, S. Sandmeyer, M. Ho, R. Murad, R. Ramirez, M. Macchietto and E. Park for many helpful discussions of this manuscript.
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Zeng, W., Mortazavi, A. Technical considerations for functional sequencing assays. Nat Immunol 13, 802–807 (2012). https://doi.org/10.1038/ni.2407
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DOI: https://doi.org/10.1038/ni.2407
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