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Chromosomal effects of adeno-associated virus vector integration

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Abstract

Adeno-associated virus (AAV) vectors are currently being used in several clinical gene-therapy trials (see the NIH OBA Human Gene Transfer Clinical Trials Database); however, little is known about the chromosomal effects of vector integration. Here we report that integrated vector proviruses are associated with chromosomal deletions and other rearrangements and are frequently located on chromosome 19 (although not at the wildtype AAV integration site).

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Figure 1: Sequences of vector-chromosome junctions.
Figure 2: Chromosome 19 integration sites.

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References

  1. Rutledge, E.A. & Russell, D.W. J. Virol. 71, 8429–8436 (1997).

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Yang, C.C. et al. J. Virol. 71, 9231–9247 (1997).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Nakai, H., Iwaki, Y., Kay, M.A. & Couto, L.B. J. Virol. 73, 5438–5447 (1999).

    CAS  PubMed  PubMed Central  Google Scholar 

  4. Kotin, R.M., Linden, R.M. & Berns, K.I. EMBO J. 11, 5071–5078 (1992).

    Article  CAS  Google Scholar 

  5. Giraud, C., Winocour, E. & Berns, K.I. J. Virol. 69, 6917–6924 (1995).

    CAS  PubMed  PubMed Central  Google Scholar 

  6. Linden, R.M., Ward, P., Giraud, C., Winocour, E. & Berns, K.I. Proc. Natl Acad. Sci. USA 93, 11288–11294 (1996).

    Article  CAS  Google Scholar 

  7. Young, S.M. Jr, McCarty, D.M., Degtyareva, N. & Samulski, R.J. J. Virol. 74, 3953–3966 (2000).

    Article  CAS  Google Scholar 

  8. Hirata, R.K. & Russell, D.W. J. Virol. 74, 4612–4620 (2000).

    Article  CAS  Google Scholar 

  9. Russell, D.W., Alexander, I.E. & Miller, A.D. Proc. Natl Acad. Sci. USA 92, 5719–5723 (1995).

    Article  CAS  Google Scholar 

  10. Alexander, I.E., Russell, D.W. & Miller, A.D. J. Virol. 68, 8282–8287 (1994).

    CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

We thank R. Dong, R. Newton and the Seattle Children's Hospital cytogenetics laboratory for technical assistance. This work was supported by grants from the Cystic Fibrosis Foundation, the March of Dimes and the National Institutes of Health.

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Authors and Affiliations

  1. Department of Medicine, University of Washington, Seattle, 98195, Washington, USA

    Daniel G. Miller, Elizabeth A. Rutledge & David W. Russell

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  1. Daniel G. Miller
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  2. Elizabeth A. Rutledge
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  3. David W. Russell
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Correspondence to David W. Russell.

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Miller, D., Rutledge, E. & Russell, D. Chromosomal effects of adeno-associated virus vector integration. Nat Genet 30, 147–148 (2002). https://doi.org/10.1038/ng824

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