Skip to main content
SpringerLink
Log in

Assembly of microarrays for genome-wide measurement of DNA copy number

  • Brief Communication
  • Published:

From Nature Genetics

View current issue Submit your manuscript

Abstract

We have assembled arrays of approximately 2,400 BAC clones for measurement of DNA copy number across the human genome. The arrays provide precise measurement (s.d. of log2 ratios=0.05–0.10) in cell lines and clinical material, so that we can reliably detect and quantify high-level amplifications and single-copy alterations in diploid, polyploid and heterogeneous backgrounds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Measurement of single-copy changes.
Figure 2: Genome-wide copy-number variation in two breast tumors.

Similar content being viewed by others

References

  1. Pinkel, D. et al. Nature Genet. 20, 207–211 (1998).

    Article  CAS  Google Scholar 

  2. Albertson, D.G. et al. Nature Genet. 25, 144–146 (2000).

    Article  CAS  Google Scholar 

  3. Klein, C.A., Schmidt-Kittler, O., Schardt, J.A., Pantel, K., Speicher, M.R. & Riethmuller, G. Proc. Natl Acad. Sci. USA 96, 4494–4499 (1999).

    Article  CAS  Google Scholar 

  4. Cheung, V.G. et al. Nature 409, 953–958 (2001).

    Article  CAS  Google Scholar 

  5. Knight, S.J.L. et al. Am. J. Hum. Genet. 67, 320–332 (2000).

    Article  CAS  Google Scholar 

  6. Geschwind, D.H. et al. Dev. Genet. 23, 215–29 (1998).

    Article  CAS  Google Scholar 

  7. Lucito, R. et al. Genome Res. 10, 1726–1736 (2000).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank J. Flint, D. Ledbetter, C. Lese and Vysis, Inc. for telomere clones and clones containing certain named genes. This work was supported by NIH grants CA80314, CA83040, CA84118, HD17665 and CA58207, by California BCRP grant 2RB-0225 and by Vysis, Inc.

Author information

Authors and Affiliations

  1. Comprehensive Cancer Center, University of California San Francisco, San Francisco, 94143, California

    Antoine M. Snijders, Richard Segraves, Stephanie Blackwood, Nils Brown, Greg Hamilton, Anna Katherine Hindle, Bing Huey, Karen Kimura, Sindy Law, Ken Myambo, Joel Palmer, Bauke Ylstra, Jingzhu Pearl Yue, Joe W. Gray, Ajay N. Jain, Daniel Pinkel & Donna G. Albertson

  2. Cancer Research Institute, University of California San Francisco, San Francisco, 94143, California

    Antoine M. Snijders, Stephanie Blackwood, Anna Katherine Hindle, Sindy Law, Joel Palmer, Bauke Ylstra, Ajay N. Jain & Donna G. Albertson

  3. Department of Laboratory Medicine, University of California San Francisco, San Francisco, 94143, California

    Joe W. Gray, Ajay N. Jain, Daniel Pinkel & Donna G. Albertson

  4. Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, 14263, New York

    Norma Nowak & Jeffrey Conroy

Authors
  1. Antoine M. Snijders
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Norma Nowak
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Richard Segraves
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Stephanie Blackwood
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nils Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jeffrey Conroy
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Greg Hamilton
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Anna Katherine Hindle
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Bing Huey
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Karen Kimura
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Sindy Law
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Ken Myambo
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Joel Palmer
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Bauke Ylstra
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Jingzhu Pearl Yue
    View author publications

    You can also search for this author in PubMed Google Scholar

  16. Joe W. Gray
    View author publications

    You can also search for this author in PubMed Google Scholar

  17. Ajay N. Jain
    View author publications

    You can also search for this author in PubMed Google Scholar

  18. Daniel Pinkel
    View author publications

    You can also search for this author in PubMed Google Scholar

  19. Donna G. Albertson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Donna G. Albertson.

Supplementary information

Rights and permissions

Reprints and permissions

About this article

Cite this article

Snijders, A., Nowak, N., Segraves, R. et al. Assembly of microarrays for genome-wide measurement of DNA copy number. Nat Genet 29, 263–264 (2001). https://doi.org/10.1038/ng754

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/ng754

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation