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A novel GC–rich human macrosatellite VNTR in Xq24 is differentially methylated on active and inactive X chromosomes

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Abstract

A new X chromosome–specific repetitive sequence, a 3 kilobase HindIII clone with a base composition of 63% C+G, has been isolated. The sequence is organized as a hypervariable tandem repeat cluster ranging in size from 150–350 kilobases, with outlying single copies. This locus, designated DXZ4 and mapped to chromosome band Xq24, may consist of as many as 50 variable–length alleles. It represents a class of variable number of tandem repeat polymorphism which may be termed ‘macrosatellite’. The cluster is highly methylated on the active X chromosome and hypomethylated on the inactive X.

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Authors and Affiliations

  1. Department of Genetics, Stanford University School of Medicine, Stanford, California, 94305, USA

    Joseph Giacalone & Uta Francke

  2. Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California, 94143, USA

    Jonathan Friedes

  3. Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, 94305, USA

    Uta Francke

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  1. Joseph Giacalone
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  2. Jonathan Friedes
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  3. Uta Francke
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Giacalone, J., Friedes, J. & Francke, U. A novel GC–rich human macrosatellite VNTR in Xq24 is differentially methylated on active and inactive X chromosomes. Nat Genet 1, 137–143 (1992). https://doi.org/10.1038/ng0592-137

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  • DOI: https://doi.org/10.1038/ng0592-137

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