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Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3

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Abstract

A total of 116, 118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.

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Authors and Affiliations

  1. Receptor Biochemistry and Molecular Biology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, 20892, USA

    A. Martin-Gallardo, W. R. McCombie, J. D. Gocayne, M. G. FitzGerald, S. Wallace, B. M. B. Lee, S. Trapp, J. M. Kelley, L-I. Liu, M. Dubnick, A. R. Kerlavage, C. Fields & J. C. Venter

  2. Human Genome Center, Lawrence Livermore National Laboratory, Livermore, California, 94551, USA

    J. Lamerdin, P. de Jong & A. Carrano

  3. Applied Biosystems, Inc., 850 Lincoln Center Drive, Foster City, California, 94404, USA

    L. A. Johnston-Dow

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  1. A. Martin-Gallardo
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  2. W. R. McCombie
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  3. J. D. Gocayne
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  4. M. G. FitzGerald
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  5. S. Wallace
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  7. J. Lamerdin
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  9. J. M. Kelley
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  10. L-I. Liu
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  11. M. Dubnick
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  12. L. A. Johnston-Dow
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  13. A. R. Kerlavage
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  14. P. de Jong
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  15. A. Carrano
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  16. C. Fields
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Martin-Gallardo, A., McCombie, W., Gocayne, J. et al. Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3. Nat Genet 1, 34–39 (1992). https://doi.org/10.1038/ng0492-34

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  • DOI: https://doi.org/10.1038/ng0492-34

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