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High-throughput identification of genetic interactions in HIV-1

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A study characterizes the in vitro replicative capacity of over 70,000 clinical isolates of HIV-1 in the absence of drugs, or in the presence of one of 15 individual drugs. The largest survey of the effects of mutations on fitness undertaken in any organism, this study finds extensive pairwise interactions among over 1,800 variable sites identified through sequencing the protease and reverse transcriptase genes.

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Figure 1: Single and higher-order epistatic interactions in HIV evolution.

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Authors and Affiliations

  1. Daniel M. Weinreich is in the Department of Ecology and Evolutionary Biology and the Center for Computational Molecular Biology at Brown University, Providence, Rhode Island, USA.,

    Daniel M Weinreich

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  1. Daniel M Weinreich
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Correspondence to Daniel M Weinreich.

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The author declares no competing financial interests.

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Weinreich, D. High-throughput identification of genetic interactions in HIV-1. Nat Genet 43, 398–400 (2011). https://doi.org/10.1038/ng.820

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