Abstract
Cellular systems allow transition-metal ions to reach or leave the cell or intracellular locations through metal transfer between proteins. By coupling mutagenesis and advanced NMR experiments, we structurally characterized the adduct between the copper chaperone Atx1 and the first copper(I)-binding domain of the Ccc2 ATPase. Copper was required for the interaction. This study provides an understanding of metal-mediated protein-protein interactions in which the metal ion is essential for the weak, reversible interaction between the partners.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnesano, F. et al. J. Biol. Chem. 276, 41365–41376 (2001).
Arnesano, F., Banci, L., Bertini, I., Huffman, D.L. & O'Halloran, T.V. Biochemistry 40, 1528–1539 (2001).
Banci, L., Bertini, I., Ciofi-Baffoni, S., Huffman, D.L. & O'Halloran, T.V. J. Biol. Chem. 276, 8415–8426 (2001).
Wernimont, A.K., Huffman, D.L., Lamb, A.L., O'Halloran, T.V. & Rosenzweig, A.C. Nat. Struct. Biol. 7, 766–771 (2000).
Tanchou, V. et al. Biochem. Biophys. Res. Commun. 325, 388–394 (2004).
Walker, J.M. et al. J. Biol. Chem. 279, 15376–15384 (2004).
van Dongen, E.M., Klomp, L.W. & Merkx, M. Biochem. Biophys. Res. Commun. 323, 789–795 (2004).
Banci, L. et al. J. Biol. Chem. 280, 38259–38263 (2005).
Banci, L. et al. FEBS J. 272, 865–871 (2005).
Banci, L. et al. J. Mol. Biol. 352, 409–417 (2005).
Larin, D. et al. J. Biol. Chem. 274, 28497–28504 (1999).
Strausak, D. et al. J. Biol. Chem. 278, 20821–20827 (2003).
Banci, L., Bertini, I., Ciofi-Baffoni, S., Del Conte, R. & Gonnelli, L. Biochemistry 42, 1939–1949 (2003).
Banci, L. et al. Proc. Natl. Acad. Sci. USA. 103, 5729–5734 (2006).
Acknowledgements
Financial support from the Ministero dell'Istruzione, dell'Università e della Ricerca (FIRB project n. RBLA032ZM7) and Ente Cassa di Risparmio di Firenze is gratefully acknowledged.
Author information
Authors and Affiliations
Contributions
L.B., I.B. and A.R. defined the experimental strategy and wrote the article. N.H. and R.P. contributed to the discussion and interpretation of the results. L.G. and P.V. produced the samples. F.C. and I.C.F. performed all spectroscopic measurements.
Corresponding author
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Banci, L., Bertini, I., Cantini, F. et al. The Atx1-Ccc2 complex is a metal-mediated protein-protein interaction. Nat Chem Biol 2, 367–368 (2006). https://doi.org/10.1038/nchembio797
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/nchembio797
- Springer Nature America, Inc.
This article is cited by
-
Human glutaredoxin-1 can transfer copper to isolated metal binding domains of the P1B-type ATPase, ATP7B
Scientific Reports (2020)
-
Using NMR spectroscopy to investigate the role played by copper in prion diseases
Neurological Sciences (2020)
-
Trapping intermediates in metal transfer reactions of the CusCBAF export pump of Escherichia coli
Communications Biology (2018)
-
Disease-causing point-mutations in metal-binding domains of Wilson disease protein decrease stability and increase structural dynamics
BioMetals (2017)
-
Enthalpy-entropy compensation at play in human copper ion transfer
Scientific Reports (2015)