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Memorizing spatiotemporal patterns

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Live samples are intrinsically highly dynamic, yet techniques to monitor these complex environments usually reflect snapshots, thus making time-lapse imaging necessary to explore temporal progression of biological functions. Recent results indicate that exploiting some basic features of fluorescent protein maturation, such as green-to-red maturation of engineered proteins, should allow probing of temporally regulated information.

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Figure 1: A comparison of schemes for the formation and maturation of chromophores in fluorescent proteins.
Figure 2: Principle of the bimolecular fluorescence complementation assay.
Figure 3

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Acknowledgements

The authors thank H. Mizuno and T. Kogure for preparation of figures and D. Mou for critical reading of the manuscript.

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Authors and Affiliations

  1. Atsushi Miyawaki is in the Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan, and the Life Function and Dynamics Project, Exploratory Research for Advanced Technology, JST, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan. matsushi@brain.riken.jp,

    Atsushi Miyawaki

  2. Satoshi Karasawa is in the Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan and Amalgaam Co., Ltd., 2-9-3 Itabashi, Itabashi-ku, Tokyo 173-0004, Japan.,

    Satoshi Karasawa

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  1. Atsushi Miyawaki
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Miyawaki, A., Karasawa, S. Memorizing spatiotemporal patterns. Nat Chem Biol 3, 598–601 (2007). https://doi.org/10.1038/nchembio1007-598

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