The parallel determination of more than 100 absolute intracellular metabolite concentrations by isotope-labeled HPLC-MS allowed a comprehensive analysis of the cellular state, its metabolic capacities and modes of regulation. Among other findings, it became obvious that for most enzymes the concentrations were above the Km values, which indicates a trend toward saturation of most enzyme active sites.
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Schomburg, D. A metabolic network described in absolute terms. Nat Chem Biol 5, 535–536 (2009). https://doi.org/10.1038/nchembio0809-535
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DOI: https://doi.org/10.1038/nchembio0809-535
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