Genetic manipulation of biosynthetic pathways is a useful method for producing analogs of complex bioactive metabolites, but this technique can be challenging when performed in the natural producer of the target compounds. Reconstruction of biosynthetic gene clusters in E. coli could be the key to rapid heterologous production of natural products and genetic manipulation of their biosynthetic pathways.
This is a preview of subscription content, log in via an institution to check access.
Katie Ris
References
Walsh, C.T. ChemBioChem 3, 124–134 (2002).
Watanabe, K. et al. Nat. Chem. Biol. 2, 423–428 (2006).
Pfeifer, B.A., Admiraal, S.J., Gramajo, H., Cane, D.E. & Khosla, C. Science 291, 1790–1792 (2001).
Watanabe, K., Rude, M.A., Walsh, C.T. & Khosla, C. Proc. Natl. Acad. Sci. USA 100, 9774–9778 (2003).
Pfeifer, B.A., Wang, C.C.C., Walsh, C.T. & Khosla, C. Appl. Environ. Microbiol. 69, 6698–6702 (2003).
Gruenewald, S., Mootz, H.D., Stehmeier, P. & Stachelhaus, T. Appl. Environ. Microbiol. 70, 3282–3291 (2004).
Peirú, S., Menzella, H.G., Rodriguez, E., Carney, J. & Gramajo, H. Appl. Environ. Microbiol. 71, 2539–2547 (2005).
König, G.M., Kehraus, S., Seibert, S.F., Abdel-Lateff, A. & Müller, D. ChemBioChem 7, 229–238 (2006).
Schmidt, E.W. et al. Proc. Natl. Acad. Sci. USA 102, 7315–7320 (2005).
Wenzel, S.C. et al. Chem. Biol. 12, 349–356 (2005).
Mutka, S.C., Carney, J.R., Liu, Y. & Kennedy, J. Biochemistry 45, 1321–1330 (2006).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Challis, G. Engineering Escherichia coli to produce nonribosomal peptide antibiotics. Nat Chem Biol 2, 398–400 (2006). https://doi.org/10.1038/nchembio0806-398
Issue Date:
DOI: https://doi.org/10.1038/nchembio0806-398
- Springer Nature America, Inc.