Skip to main content
SpringerLink
Log in

Peering inside the black box to find enzyme-bound intermediates

  • News & Views
  • Published:

From Nature Chemical Biology

View current issue Submit your manuscript

The NMR structures of three intermediates bound to the pyridoxal-synthesizing enzyme Pdx1 illuminate its complex catalytic cycle. The success of this approach with a high-molecular-weight protein should encourage further applications of NMR techniques for characterizing enzyme reaction pathways.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: The overall reaction mediated by Pdx1 and Pdx2 to combine R5P, G3P and glutamine to synthesize PLP.

References

  1. Hanes, J.W., Keresztes, I. & Begley, T.P. Nat. Chem. Biol. 4, 425–430 (2008).

    Article  CAS  Google Scholar 

  2. Townsend, C.A. Curr. Opin. Chem. Biol. 6, 583–589 (2002).

    Article  CAS  Google Scholar 

  3. Gerratana, B., Arnett, S.O., Stapon, A. & Townsend, C.A. Biochemistry 43, 15936–15945 (2004).

    Article  CAS  Google Scholar 

  4. Hanes, J.W., Keresztes, I. & Begley, T.G. Angew. Chem. Int. Ed. 47, 2102–2105 (2008).

    Article  CAS  Google Scholar 

  5. Schwab, J.M., Ho, C.-k., Li, W.-b., Townsend, C.A. & Salituro, G.M. J. Am. Chem. Soc. 108, 5309–5316 (1986).

    Article  CAS  Google Scholar 

  6. Scott, A.I. J. Org. Chem. 68, 2529–2539 (2003).

    Article  CAS  Google Scholar 

  7. Battersby, A.R. Nat. Prod. Rep. 17, 507–526 (2000).

    Article  CAS  Google Scholar 

  8. Anderson, K.S. et al. Biochemistry 29, 1460–1465 (1990).

    Article  CAS  Google Scholar 

  9. Dorrestein, P.C. & Kelleher, N.L. Nat. Prod. Rep. 23, 893–918 (2006).

    Article  CAS  Google Scholar 

  10. Crawford, J.M. et al. Science 320, 243–246 (2008).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Craig Townsend is in the Department of Chemistry at The Johns Hopkins University and has joint appointments in the Departments of Biology and Biophysics, 252 Remsen Hall, 3400 North Charles Street, Baltimore, Maryland 21218, USA. ctownsend@jhu.edu,

    Craig Townsend

Authors
  1. Craig Townsend
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Townsend, C. Peering inside the black box to find enzyme-bound intermediates. Nat Chem Biol 4, 390–391 (2008). https://doi.org/10.1038/nchembio0708-390

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nchembio0708-390

  • Springer Nature America, Inc.

Search

Navigation