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GPCR–G protein preassembly?

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The extent to which ligand-free GPCRs exist in quasi-stable 'precoupled' complexes with G proteins in vivo is uncertain. New research, using fluorescence recovery after photobleaching (FRAP), reveals a structural requirement for and functional consequences of muscarinic acetylcholine receptor–Gq protein preassembly.

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Figure 1: Using FRAP to monitor GPCR–G protein preassembly.

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Authors and Affiliations

  1. R.A. John Challiss is in the Department of Cell Physiology & Pharmacology, College of Medicine, Biological Sciences & Psychology, University of Leicester, Leicester, UK.,

    R A John Challiss

  2. Jürgen Wess is in the Molecular Signaling Section, Laboratory of Bioorganic Chemistry, National Institutes of Health–National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland, USA.,

    Jürgen Wess

Authors
  1. R A John Challiss
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  2. Jürgen Wess
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Correspondence to R A John Challiss or Jürgen Wess.

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The authors declare no competing financial interests.

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Challiss, R., Wess, J. GPCR–G protein preassembly?. Nat Chem Biol 7, 657–658 (2011). https://doi.org/10.1038/nchembio.665

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