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Protein Design

Getting to the bottom of the TIM barrel

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Natural (βα)8-barrel proteins support diverse catalytic functions and are fertile scaffolds for engineering synthetic enzymes. The atomic-resolution structure determination of a computationally guided, de novo–designed symmetric barrel is a long-awaited advance that opens up new opportunities for enzyme design.

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Figure 1: A four-fold-symmetric (βα)8-barrel was designed by constraining key aspects of the topology of the core (βα)2 unit and then using computational sequence design.

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Authors and Affiliations

  1. Vikas Nanda is a Resident Faculty Member at the Center for Advanced Biotechnology and Medicine, Robert Wood Johnson Medical School, Rutgers University, Piscataway, New Jersey, United States.,

    Vikas Nanda

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  1. Vikas Nanda
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Correspondence to Vikas Nanda.

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Nanda, V. Getting to the bottom of the TIM barrel. Nat Chem Biol 12, 2–3 (2016). https://doi.org/10.1038/nchembio.1987

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