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Cytochrome bd confers nitric oxide resistance to Escherichia coli

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Abstract

The aerobic respiratory chain of Escherichia coli has two terminal quinol oxidases: cytochrome bo and cytochrome bd. Cytochrome bd was thought to function solely to facilitate micro-aerobic respiration. However, it has recently been shown to be overexpressed under conditions of nitric oxide (NO) stress; we show here that cytochrome bd is crucial for protecting E. coli cells from NO-induced growth inhibition by virtue of its fast NO dissociation rate.

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Figure 1: Nitric oxide and oxygen sensitivity of cytochromes bo and bd in E.coli.

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References

  1. Gennis, R.B. & Stewart, V. in Respiration Vol.1 (eds. Curtiss, R. et al.) 217–261 (American Society for Microbiology Press, Washington, DC, 1996).

    Google Scholar 

  2. van der Oost, J. et al. FEMS Microbiol. Lett. 121, 1–9 (1994).

    Article  CAS  Google Scholar 

  3. Rothery, R.A. & Ingledew, W.J. Biochem. J. 261, 437–443 (1989).

    Article  CAS  Google Scholar 

  4. Puustinen, A., Finel, M., Haltia, T., Gennis, R.B. & Wikstrom, M. Biochemistry 30, 3936–3942 (1991).

    Article  CAS  Google Scholar 

  5. Calhoun, M.W., Oden, K.L., Gennis, R.B., de Mattos, M.J. & Neijssel, O.M. J. Bacteriol. 175, 3020–3025 (1993).

    Article  CAS  Google Scholar 

  6. Cotter, P.A., Chepuri, V., Gennis, R.B. & Gunsalus, R.P. J. Bacteriol. 172, 6333–6338 (1990).

    Article  CAS  Google Scholar 

  7. Rice, C.W. & Hempfling, W.P. J. Bacteriol. 134, 115–124 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Alexeeva, S., Hellingwerf, K.J. & Teixeira de Mattos, M.J. J. Bacteriol. 184, 1402–1406 (2002).

    Article  CAS  Google Scholar 

  9. Pullan, S.T. et al. J. Bacteriol. 189, 1845–1855 (2007).

    Article  CAS  Google Scholar 

  10. Hyduke, D.R., Jarboe, L.R., Tran, L.M., Chou, K.J. & Liao, J.C. Proc. Natl. Acad. Sci. USA 104, 8484–8489 (2007).

    Article  CAS  Google Scholar 

  11. Yu, H. et al. FEBS Lett. 409, 161–165 (1997).

    Article  CAS  Google Scholar 

  12. Verkhovsky, M.I., Morgan, J.E., Puustein, A. & Wikstrom, M. Nature 380, 268–270 (1996).

    Article  CAS  Google Scholar 

  13. Junemann, S. & Wrigglesworth, J.M. J. Biol. Chem. 270, 16213–16220 (1995).

    Article  CAS  Google Scholar 

  14. Borisov, V.B. et al. FEBS Lett. 576, 201–204 (2004).

    Article  CAS  Google Scholar 

  15. Sarti, P. et al. Biochem. Biophys. Res. Commun. 274, 183–187 (2000).

    Article  CAS  Google Scholar 

  16. Cooper, C.E. Biochim. Biophys. Acta 1411, 290–309 (1999).

    Article  CAS  Google Scholar 

  17. Borisov, V.B. et al. Biochem. Biophys. Res. Commun. 355, 97–102 (2007).

    Article  CAS  Google Scholar 

  18. Cary, S.P., Winger, J.A. & Marletta, M.A. Proc. Natl. Acad. Sci. USA 102, 13064–13069 (2005).

    Article  CAS  Google Scholar 

  19. Rinaldo, S., Arcovito, A., Brunori, M. & Cutruzzola, F. J. Biol. Chem. 282, 14761–14767 (2007).

    Article  CAS  Google Scholar 

  20. Gardner, A.M. & Gardner, P.R. J. Biol. Chem. 277, 8166–8171 (2002).

    Article  CAS  Google Scholar 

  21. Gardner, A.M., Gessner, C.R. & Gardner, P.R. J. Biol. Chem. 278, 10081–10086 (2003).

    Article  CAS  Google Scholar 

  22. Gardner, A.M., Martin, L.A., Gardner, P.R., Dou, Y. & Olson, J.S. J. Biol. Chem. 275, 12581–12589 (2000).

    Article  CAS  Google Scholar 

  23. Cooper, C.E. et al. Biochim. Biophys. Acta 1607, 27–34 (2003).

    Article  CAS  Google Scholar 

  24. Blackmore, R.S., Greenwood, C. & Gibson, Q.H. J. Biol. Chem. 266, 19245–19249 (1991).

    CAS  PubMed  Google Scholar 

  25. Mason, M.G., Nicholls, P., Wilson, M.T. & Cooper, C.E. Proc. Natl. Acad. Sci. USA 103, 708–713 (2006).

    Article  CAS  Google Scholar 

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Acknowledgements

We thank the Wellcome Trust (069769) and the Biotechnology and Biological Sciences Research Council (BB/D017858/1) for financial support.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK

    Maria G Mason, Peter Nicholls, Paul S Dobbin & Chris E Cooper

  2. Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK

    Mark Shepherd, Kathryn S Dodsworth & Robert K Poole

Authors
  1. Maria G Mason
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  2. Mark Shepherd
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  3. Peter Nicholls
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  4. Paul S Dobbin
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  5. Kathryn S Dodsworth
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  6. Robert K Poole
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  7. Chris E Cooper
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Contributions

M.G.M., P.S.D. and C.E.C. designed the experiments; M.G.M., M.S. and K.S.D. performed the experiments; M.G.M., M.S., P.N., K.S.D., R.K.P. and C.E.C. analyzed the data; and M.G.M., M.S., R.K.P. and C.E.C. wrote the paper.

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Correspondence to Maria G Mason.

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Supplementary Figures 1–3 and Supplementary Methods (PDF 383 kb)

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Mason, M., Shepherd, M., Nicholls, P. et al. Cytochrome bd confers nitric oxide resistance to Escherichia coli. Nat Chem Biol 5, 94–96 (2009). https://doi.org/10.1038/nchembio.135

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