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Torqueing about pores

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Cryo-EM, crystallography, biochemical experiments and computational approaches have been used to study different intermediate states of the Aeromonas hydrophila toxin aerolysin en route to pore formation. These results reveal that an unexpected and marked rotation of the core aerolysin machinery is required to unleash the membrane-spanning regions.

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Figure 1: Figure showing the movement from prepore to pore.

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Authors and Affiliations

  1. James C. Whisstock is in the Department of Biochemistry and Molecular Biology and the Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, Clayton, Melbourne, Victoria, Australia.,

    James C Whisstock

  2. Michelle A. Dunstone is in the Department of Biochemistry and Molecular Biology and the Department of Microbiology, Monash University, Clayton, Melbourne, Victoria, Australia.,

    Michelle A Dunstone

Authors
  1. James C Whisstock
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  2. Michelle A Dunstone
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Correspondence to James C Whisstock.

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The authors declare no competing financial interests.

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Whisstock, J., Dunstone, M. Torqueing about pores. Nat Chem Biol 9, 605–606 (2013). https://doi.org/10.1038/nchembio.1341

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