Analysing post-translational modifications of histone proteins as they occur within chromatin is challenging due to their large number and chemical diversity. A major step forward has now been achieved by using split intein chemistry to engineer functionalized histones within cells.
References
Simon, M. D. et al. Cell 128, 1003–1012 (2007).
Shogren-Knaak, M. et al. Science 311, 844–847 (2006).
McGinty, R. K., Kim, J., Chatterjee, C., Roeder, R. G. & Muir, T. W. Nature 453, 812–816 (2008).
David, Y., Vila-Perelló, M., Verma, S. & Muir, T. W. Nature Chem. 7, 394–402 (2015).
Giriat, I. & Muir, T. W. J. Am. Chem. Soc. 125, 7180–7181 (2003).
Thiel, I. V., Volkmann, G., Pietrokovski, S. & Mootz, H. D. Angew. Chem. Int. Ed. 53, 1306–1310 (2014).
Carvajal-Vallejos, P., Pallisse, R., Mootz, H. D. & Schmidt, S. R. J. Biol. Chem. 287, 28686–28696 (2012).
Neumann, H. et al. Mol. Cell 36, 153–163 (2009).
Nguyen, D. P., Garcia Alai, M. M., Kapadnis, P. B., Neumann, H. & Chin, J. W. J. Am. Chem. Soc. 131, 14194–14195 (2009).
Wilkins, B. J. et al. Science 343, 77–80 (2014).
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Fischle, W., Schwarzer, D. & Mootz, H. Chromatin chemistry goes cellular. Nature Chem 7, 371–373 (2015). https://doi.org/10.1038/nchem.2249
Published:
Issue Date:
DOI: https://doi.org/10.1038/nchem.2249
- Springer Nature Limited