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Directionality of F-actin cables changes during the fission yeast cell cycle

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Abstract

Longitudinal F-actin cables are thought to be important for transporting materials for polarized cell growth in fission yeast. We show that most F-actin in the cables is oriented such that the barbed end faces the nearest cell tip during interphase; however, this directionality is reversed during mitosis. These orientations of F-actin ensure proper transport of materials to growing sites during these cell-cycle stages.

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Figure 1: Ultrastructure of F-actin cables and orientation of F-actin in cdc25 cells.
Figure 2: Recovery of cables after Lat-A treatment.

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Acknowledgements

We thank J. Usukura and M. Sameshima for help in determining the directionality of F-actin. This study was supported by grants from the Ministry of Education, Science and Culture of Japan.

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Author notes

  1. Tomoko Kamasaki

    Present address: Department of Oral Histology, School of Dentistry, Showa University, Tokyo, 142-8555, Japan

  2. Ritsuko Arai

    Present address: Department of Chemical Genetics, Riken, Wako, 351-0198, Japan

Authors and Affiliations

  1. Division of Biology, Graduate School of Arts and Sciences, University of Tokyo, Tokyo, 153-8902, Japan

    Tomoko Kamasaki, Ritsuko Arai & Issei Mabuchi

  2. Division of Material and Biological Function Sciences, Graduate School of Science, Japan Women's University, Tokyo, 112-8681, Japan

    Tomoko Kamasaki & Masako Osumi

  3. Instistute of Medical Mycology, Teikyo University, Tokyo, 192-0395, Japan

    Masako Osumi

Authors
  1. Tomoko Kamasaki
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  2. Ritsuko Arai
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  3. Masako Osumi
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  4. Issei Mabuchi
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Corresponding author

Correspondence to Issei Mabuchi.

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The authors declare no competing financial interests.

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Supplementary Information

Supplementary figures S1 and S2, supplementary table S1 and supplementary methods (PDF 344 kb)

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Kamasaki, T., Arai, R., Osumi, M. et al. Directionality of F-actin cables changes during the fission yeast cell cycle. Nat Cell Biol 7, 916–917 (2005). https://doi.org/10.1038/ncb1295

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