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Arp2/3 ATP hydrolysis: to branch or to debranch?

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Actin filament branch initiation by the Arp2/3 complex requires that the Arp2 and Arp3 subunits are loaded with ATP. However, whether ATP hydrolysis is required for branch initiation or subsequent debranching is controversial. New analysis of budding yeast Arp2/3 mutants suggests that debranching, not branching, depends on ATP hydrolysis.

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Figure 1: Schematic representation of contrasting models for the role of ATP hydrolysis in Arp2/3 complex-mediated actin-filament branching and debranching.

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  1. Departments of Molecular Genetics and Cell Biology and of Biochemistry and Molecular Biology, The University of Chicago, Chicago, 60637, IL, USA

    David R. Kovar

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  1. David R. Kovar
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Kovar, D. Arp2/3 ATP hydrolysis: to branch or to debranch?. Nat Cell Biol 8, 783–785 (2006). https://doi.org/10.1038/ncb0806-783

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