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Automated de novo protein sequencing of monoclonal antibodies

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Figure 1: Protein contigs resulting from shotgun protein sequencing of aBTLA tandem mass spectra.
Figure 2: CSPS methodology.

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Acknowledgements

The authors are grateful to Dan Eaton, Jill Calemine-Fenaux, Ari Frank, Richard Vandlen, Oleg Borisov and Bao-Jen Shyong for help with various aspects of this manuscript. This project was supported by National Institutes of Health grants NIGMS 1-R01-RR16522 and 1-P41-RR024851-01.

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Authors and Affiliations

  1. Department of Computer Science and Engineering, University of California, San Diego, 92093, California, USA

    Nuno Bandeira & Pavel Pevzner

  2. Protein Chemistry Department, Genentech Inc., 1 DNA Way, South San Francisco, 94080, California, USA

    Victoria Pham, David Arnott & Jennie R Lill

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  1. Nuno Bandeira
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  2. Victoria Pham
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  3. Pavel Pevzner
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  4. David Arnott
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  5. Jennie R Lill
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Competing interests

D.A., V.P. and J.R.L. are employed by Genentech, Inc., a pharmaceutical company.

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Bandeira, N., Pham, V., Pevzner, P. et al. Automated de novo protein sequencing of monoclonal antibodies. Nat Biotechnol 26, 1336–1338 (2008). https://doi.org/10.1038/nbt1208-1336

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