By combining a refined Agrobacterium tumefaciens–mediated transformation protocol with stringent selection, researchers have succeeded in isolating gene-targeted rice plants with surprisingly high efficiency.
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Shimamoto, K. Picking genes in the rice genome. Nat Biotechnol 20, 983–984 (2002). https://doi.org/10.1038/nbt1002-983
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DOI: https://doi.org/10.1038/nbt1002-983
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