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A synthetic DNA transplant

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The complete set of tools needed to synthesize a functional genome and transplant it into a mycoplasma cell opens up the possibility of mixing and matching natural and synthetic DNA to make genomes with new capabilities.

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Figure 1: Simplified protocol used to produce Mycoplasma mycoides JCVI-syn1.0 using a chassis derived from M. capricolum and a 1.08 Mbp variant of the M. mycoides genome designed to carry distinguishable 'watermark' sequences.

References

  1. Gibson, D. et al. Science published online, doi: 10.1126/science.1190719 (20 May 2010).

  2. Gibson, D. et al. Science 319, 1215–1220 (2008).

    Article  CAS  Google Scholar 

  3. Lartigue, C. et al. Science 317, 632–638 (2007).

    Article  CAS  Google Scholar 

  4. Gibson, D. et al. Nat. Methods 6, 343–345 (2009).

    Article  CAS  Google Scholar 

  5. Carr, P.A. & Church, G.M. Nat. Biotechnol. 27, 1151–1162 (2009).

    Article  CAS  Google Scholar 

  6. Carlson, R. Nat. Biotechnol. 27, 1091–1094 (2009).

    Article  CAS  Google Scholar 

  7. Glass, J. et al. Proc. Natl. Acad. Sci. USA 103, 425–430 (2006).

    Article  CAS  Google Scholar 

  8. Colwell, R.R. & Grimes, D.J. (eds.) Nonculturable Microorganisms in the Environment (ASM Press, Washington, DC, 2000).

    Book  Google Scholar 

  9. Itaya, M., Tsuge, K., Koizumi, M. & Fujita, K. Proc. Natl. Acad. Sci. USA 102, 15971–15976 (2005).

    Article  CAS  Google Scholar 

  10. Takahashi, K. & Yamanaka, S. Cell 126, 663–676 (2006).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Mitsuhiro Itaya is at the Laboratory of Genome Design Biology, Institute for Advanced Biosciences, Keio University, Yamagata, Japan.,

    Mitsuhiro Itaya

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  1. Mitsuhiro Itaya
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Correspondence to Mitsuhiro Itaya.

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The author declares no competing financial interests.

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Itaya, M. A synthetic DNA transplant. Nat Biotechnol 28, 687–689 (2010). https://doi.org/10.1038/nbt0710-687

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