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Targeted mutagenesis in zebrafish

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Directed gene knockout in a vertebrate has been achieved using zinc-finger nucleases.

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Figure 1: Targeted mutagenesis of zebrafish genes with zinc-finger nucleases (ZFNs).

Katie Ris-Vicari

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Authors and Affiliations

  1. Ian G. Woods and Alexander F. Schier are in the Department of Molecular and Cellular Biology, Broad Institute, Center for Brain Science, Harvard Stem Cell Institute, Harvard University, 16 Divinity Avenue, Cambridge, Massachusetts 02138, USA. schier@fas.harvard.edu or ianwoods@mcb.harvard.edu,

    Ian G Woods & Alexander F Schier

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  1. Ian G Woods
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  2. Alexander F Schier
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Woods, I., Schier, A. Targeted mutagenesis in zebrafish. Nat Biotechnol 26, 650–651 (2008). https://doi.org/10.1038/nbt0608-650

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