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Reply to Oversulfated chondroitin sulfate is not the sole contaminant in heparin

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Figure 1: Comparison of the proton NMR spectra of synthesized OSCS at pH 7 and pH 5.
Figure 2: NMR spectra of sulfated chondroitins.
Figure 3: Two-dimensional HSQC analysis of a 10% wt/wt mixture of sulfated chondroitin (ds = 3.1) in heparin compared to a reference standard of sulfated chondroitin.
Figure 4: Two-dimensional HSQC analysis of oversulfated heparin waste compared to authentic heparin.

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Acknowledgements

The authors thank G. Cassinelli, M. Nasr and L. Buhse for discussions and the US National Institutes of Health for grant HL101721 to R.S. and R.J.L. to support this work.

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Authors and Affiliations

  1. The Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA

    Jing Pan, Yi Qian, Xiaodong Zhou, Andrew Pazandak, Sarah B Frazier, Peter Weiser, Hong Lu & Lijuan Zhang

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  1. Jing Pan
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Correspondence to Lijuan Zhang.

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Competing interests

R.S. is a director of Momenta Pharmaceuticals, Z.S. reports receiving consulting fees from Momenta Pharmaceuticals and R.J.L. is a paid consultant for Baxter Pharmaceuticals.

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Pan, J., Qian, Y., Zhou, X. et al. Reply to Oversulfated chondroitin sulfate is not the sole contaminant in heparin. Nat Biotechnol 28, 207–211 (2010). https://doi.org/10.1038/nbt0310-207

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