Skip to main content
Log in

Oversulfated chondroitin sulfate is not the sole contaminant in heparin

  • Correspondence
  • Published:

From Nature Biotechnology

View current issue Submit your manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Figure 1: Galactosamine-containing GAGs in heparin.
Figure 2: Proton chemical shifts of oversulfated heparan sulfate were eliminated in the presence of heparin.

References

  1. Guerrini, M. et al. Nat. Biotechnol. 26, 669–675 (2008).

    Article  CAS  Google Scholar 

  2. Guerrini, M. et al. Proc. Natl. Acad. Sci. USA 106, 16956–16961 (2009).

    Article  CAS  Google Scholar 

  3. Kishimoto, T.K. et al. N. Engl. J. Med. 358, 2457–2467 (2008).

    Article  CAS  Google Scholar 

  4. Hovingh, P., Piepkorn, M. & Linker, A. Biochem. J. 237, 573–581 (1986).

    Article  CAS  Google Scholar 

  5. Acostamadiedo, J.M., Iyer, U.G. & Owen, J. Expert Opin. Pharmacother. 1, 803–814 (2000).

    Article  CAS  Google Scholar 

  6. Anonymous. Danaparoid. http://en.wikipedia.org/wiki/Orgaran (accessed 17 February 2010).

  7. Frazier, S.B., Roodhouse, K., Hourcade, D.E. & Zhang, L. Open Glycosci. 1, 31–39 (2008).

    Article  CAS  Google Scholar 

  8. Studelska, D.R., Giljum, K., McDowell, L.M. & Zhang, L. Glycobiology 16, 65–72 (2006).

    Article  CAS  Google Scholar 

  9. Volpi, N., Maccari, F. & Linhardt, R.J. Anal. Biochem. 388, 140–145 (2009).

    Article  CAS  Google Scholar 

  10. Zhang, Z. et al. J. Pharm. Sci. 98, 4017–4026 (2009).

    Article  CAS  Google Scholar 

  11. Liu, H., Zhang, Z. & Linhardt, R.J. Nat. Prod. Rep. 26, 313–321 (2009).

    Article  CAS  Google Scholar 

  12. Li, F. et al. Glycoconj. J. 25, 603–610 (2008).

    Article  Google Scholar 

  13. Fareed, J. et al. Int. Angiol. 27, 457–461 (2008).

    CAS  PubMed  Google Scholar 

  14. Volpi, N. J. Chromatogr. B Biomed. Appl. 685, 27–34 (1996).

    Article  CAS  Google Scholar 

  15. Warda, M., Gouda, E.M., Toida, T., Chi, L. & Linhardt, R.J. Comp. Biochem. Physiol. C Toxicol. Pharmacol. 136, 357–365 (2003).

    Article  Google Scholar 

  16. Pan, J. et al. Glyobiol. Insights 2, 1–12 (2010).

    Article  CAS  Google Scholar 

  17. Maruyama, T., Toida, T., Imanari, T., Yu, G. & Linhardt, R.J. Carbohydr. Res. 306, 35–43 (1998).

    Article  CAS  Google Scholar 

  18. Toida, T. et al. Int. J. Biol. Macromol. 26, 233–241 (1999).

    Article  CAS  Google Scholar 

  19. Blossom, D.B. et al. N. Engl. J. Med. 359, 2674–2684 (2008).

    Article  CAS  Google Scholar 

  20. Ahmad, S. Front. Biosci. 12, 3312–3320 (2007).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to thank H. Ye and J. Reepmeyer of the FDA for purification of heparin contaminants and capillary electrophoresis analysis of heparin samples. The authors would like to thank A. d'Avignon of Washington University for the analysis of samples by NMR. The authors also thank J. Esko, E. Conrad, S. Kornfeld, J. Baenziger and D. Tollefsen for their suggestions, comments and critiques of the manuscript. L.Z. thanks E. Unanue, S. Santoro, J. Ladenson and N. Brown for their support in establishing a GAG structure/function laboratory at Washington University. L.Z. thanks J. Metz for critical reading of the original manuscript. This work is supported in part by US National Institutes of Health grant R01GM069968 to L.Z. and a St. Louis Children's Discovery Institute Research Fund to L.Z.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lijuan Zhang.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Supplementary information

Supplementary Figures, Tables and Methods

Supplementary Figures 1–5, Supplementary Table 1, and Supplementary Materials and Methods (PDF 720 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pan, J., Qian, Y., Zhou, X. et al. Oversulfated chondroitin sulfate is not the sole contaminant in heparin. Nat Biotechnol 28, 203–207 (2010). https://doi.org/10.1038/nbt0310-203

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0310-203

  • Springer Nature America, Inc.

This article is cited by

Navigation