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Sequence-activity relationships guide directed evolution

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A new method for in vitro evolution integrates computational analysis and experimental screening.

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Figure 1: Mathematical analysis bolsters directed evolution.

References

  1. Fox, R.J. et al. Nat. Biotechnol. 25, 338–344 (2007).

    Article  CAS  Google Scholar 

  2. Hibbert, E.G. et al. Biomol. Eng. 22, 11–19 (2005).

    Article  CAS  Google Scholar 

  3. Dwyer, M.A., Looger, L.L. & Hellinga, H.W. Science 304, 1967–1971 (2004).

    Article  CAS  Google Scholar 

  4. Oelschlaeger, P. & Mayo, S.L. J. Mol. Biol. 350, 395–401 (2005).

    Article  CAS  Google Scholar 

  5. Zanghellini, A. et al. Protein Sci. 15, 2785–2794 (2006).

    Article  CAS  Google Scholar 

  6. Fox, R. J. Theor. Biol. 234, 187–199 (2005).

    Article  CAS  Google Scholar 

  7. Gustafsson, C., Govindarajan, S. & Minshull, J. Curr. Opin. Biotechnol. 14, 366–370 (2003).

    Article  CAS  Google Scholar 

  8. Hayes, R.J. et al. Proc. Natl. Acad. Sci. USA 99, 15926–15931 (2002).

    Article  CAS  Google Scholar 

  9. Rothman, S.C. & Kirsch, J.F. J. Mol. Biol. 327, 593–608 (2003).

    Article  CAS  Google Scholar 

  10. Aita, T., Iwakura, M. & Husimi, Y. Protein Eng. 14, 633–638 (2001).

    Article  CAS  Google Scholar 

  11. Schmitzer, A.R., Lépine, F. & Pelletier, J.N. Protein Eng. Des. Sel. 17, 809–819 (2004).

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Joelle N. Pelletier is at the Département de chimie and Département de biochimie, H4P 2R2 and Département de chimie, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Montréal, Québec, Canada, H3C 3J7 and Robert Lortie is at the Biotechnology Research Institute, National Research Council, 6100 Royalmount Avenue, Montréal, Québec, Canada, Université de Montréal. joelle.pelletier@umontreal.ca or robert.lortie@cnrc-nrc.gc.ca,

    Joelle N Pelletier & Robert Lortie

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  1. Joelle N Pelletier
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  2. Robert Lortie
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The authors declare no competing financial interests.

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Pelletier, J., Lortie, R. Sequence-activity relationships guide directed evolution. Nat Biotechnol 25, 297–298 (2007). https://doi.org/10.1038/nbt0307-297

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