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Automated Laser-Fluorescence Sequencing

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References

  1. Ansorge, W., Sproat, B.S., Stegemann, J. and Schwager, C. 1986. A non-radioactive automated method for DNA sequence determination. J. Biochem. Biophys. Meth. 13: 315.

    Article  CAS  Google Scholar 

  2. Ansorge, W., Sproat, B.S., Stegemann, J., Schwager, C. and Zenke, M. 1987. Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis. Nucl. Acids Res. 15: 4593.

    Article  CAS  Google Scholar 

  3. Hultman, T., Ståhl, S., Hornes, E. and Uhlén, M. 1989. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucl. Acids Res. 17: 4937.

    Article  CAS  Google Scholar 

  4. Voss, H., Schwager, C., Wirkner, U., Sproat, B., Zimmerman, J., Rosenthal, A., Erfle, H., Stegemann, J. and Ansorge, W. 1989. Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA. Nucl. Acids Res. 17: 2517.

    Article  CAS  Google Scholar 

  5. Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. 74: 5463.

    Article  CAS  Google Scholar 

  6. Maxam, A.M. and Gilbert, W. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 101: 499.

    Article  Google Scholar 

  7. Kristensen, T., Voss, H. and Ansorge, W. 1987. A simple and rapid preparation of M13 sequencing templates for manual and automated dideoxy sequencing. Nucl. Acids Res. 15: 5507.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Pharmacia LKB Biotechnology, 800 Centennial Ave., Piscataway, NJ, 08854

    Mark Freeman, Carla Baehler & Steven Spotts

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  1. Mark Freeman
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  2. Carla Baehler
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  3. Steven Spotts
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Freeman, M., Baehler, C. & Spotts, S. Automated Laser-Fluorescence Sequencing. Nat Biotechnol 8, 147–148 (1990). https://doi.org/10.1038/nbt0290-147

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  • DOI: https://doi.org/10.1038/nbt0290-147

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