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Automating the Purification and Isolation of Synthetic DNA

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Abstract

We describe the application of robotics to the purification and isolation of synthetic oligodeoxyribonucleotides. Starting with commercially available components, we have designed and programmed a robot based system that can isolate oligonucleotides from polyacrylamide gel slices, prepare oligonucleotides for purification by polyacrylamide gel electrophoresis and execute a rapid purification protocol on a disposable C–18 reverse phase column. Each operation has been fully automated, including evaporative centrifugation, such that the system can execute any two procedures simultaneously with a throughput of 12 samples in about six hours.

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Authors and Affiliations

  1. Genetics Institute, Inc., 87 CambridgePark Drive, Cambridge, MA, 02140

    Simon S. Jones, John E. Brown, Darlene A. Vanstone, David K. Stone & Eugene L. Brown

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  1. Simon S. Jones
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  2. John E. Brown
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  3. Darlene A. Vanstone
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  4. David K. Stone
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  5. Eugene L. Brown
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Jones, S., Brown, J., Vanstone, D. et al. Automating the Purification and Isolation of Synthetic DNA. Nat Biotechnol 5, 67–70 (1987). https://doi.org/10.1038/nbt0187-67

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  • DOI: https://doi.org/10.1038/nbt0187-67

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