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Super-resolution microscopy writ large

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Improved expansion microscopy provides a super-resolution method that is accessible to any laboratory with a fluorescence microscope.

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Figure 1: Concept and workflow of refined expansion microscopy techniques.

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Acknowledgements

The authors thank the Neurobiology Course at the Marine Biological Laboratory for being their summer home for many years. T.M.'s laboratory is supported by the Deutsche Forschungsgemeinschaft (DFG) through the Munich Center for Systems Neurology (SyNergy; EXC 1010), the Center for Integrated Protein Science Munich (CIPSM, EXC 114), Collaborative Research Center 870, Priority Program 1710 and DFG Research Grant (MI 674/7-1). Further support came from the European Research Council under the European Union's Seventh Framework Program (FP/2007-2013; ERC Grant Agreement n. 616791) and the German-Israeli Foundation. T.M. is also associated with the German Center for Neurodegenerative Diseases (DZNE Munich). P.E. was supported by the DFG Research Training Group 1373 and the Graduate School of the Technische Universität München (TUM-GS); C.F. is supported by the Graduate School of Systemic Neuroscience (GSN, GSC 82).

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  1. Peter Engerer, Caroline Fecher and Thomas Misgeld are at the Institute of Neuronal Cell Biology, Munich, Germany, the Center of Integrated Protein Science (CIPSM), Munich, Germany, the German Center for Neurodegenerative Diseases (DZNE), Munich, Germany, and the Munich Cluster of Systems Neurology (SyNergy), Munich, Germany.,

    Peter Engerer, Caroline Fecher & Thomas Misgeld

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  1. Peter Engerer
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  2. Caroline Fecher
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  3. Thomas Misgeld
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Correspondence to Thomas Misgeld.

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Engerer, P., Fecher, C. & Misgeld, T. Super-resolution microscopy writ large. Nat Biotechnol 34, 928–930 (2016). https://doi.org/10.1038/nbt.3669

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