Short-read sequencing provides haplotype information when long DNA fragments are barcoded in microfluidic droplets.
References
Zheng, G.X.Y. et al. Nat. Biotechnol. 34, 310–318 (2016).
Kitzman, J.O. et al. Sci. Transl. Med. 4, 137ra76 (2012).
Fan, H.C. et al. Nature 487, 320–324 (2012).
Jiang, P. et al. Proc. Natl. Acad. Sci. USA 112, E1317–E1325 (2015).
Tewhey, R., Bansal, V., Torkamani, A., Topol, E.J. & Schork, N.J. Nat. Rev. Genet. 12, 215–223 (2011).
Browning, S.R. & Browning, B.L. Nat. Rev. Genet. 12, 703–714 (2011).
Snyder, M.W., Adey, A., Kitzman, J.O. & Shendure, J. Nat. Rev. Genet. 16, 344–358 (2015).
Stephens, P.J. et al. Cell 144, 27–40 (2011).
Kitzman, J.O. et al. Nat. Biotechnol. 29, 59–63 (2011).
Chaisson, M.J.P. et al. Nature 517, 608–611 (2015).
Kuleshov, V. et al. Nat. Biotechnol. 34, 64–69 (2016).
Sharon, D., Tilgner, H., Grubert, F. & Snyder, M. Nat. Biotechnol. 31, 1009–1014 (2013).
Klein, A.M. et al. Cell 161, 1187–1201 (2015)
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Kitzman, J. Haplotypes drop by drop. Nat Biotechnol 34, 296–298 (2016). https://doi.org/10.1038/nbt.3500
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DOI: https://doi.org/10.1038/nbt.3500
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