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Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems

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Figure 1: Targeted disruption in the Tet gene loci in rat by means of CRISPR-Cas systems.

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Acknowledgements

This study was supported by grants from the National Basic Research Program of China 2012CBA01300, “Strategic Priority Research Program” of the Chinese Academy of Sciences XDA01020101, the National Science Foundation of China 90919060 and the Ministry of Science and Technology of China 2011CBA01101 (all to Q.Z.).

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Author notes

  1. Wei Li and Fei Teng: These authors contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China

    Wei Li, Fei Teng, Tianda Li & Qi Zhou

  2. University of Chinese Academy of Sciences, Beijing, China

    Fei Teng

Authors
  1. Wei Li
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  2. Fei Teng
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  3. Tianda Li
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  4. Qi Zhou
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Correspondence to Qi Zhou.

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Supplementary Methods, Supplementary Discussion, Supplementary Figures 1–9, Supplementary Tables 1–3 and Supplementary Sequences (PDF 3420 kb)

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Li, W., Teng, F., Li, T. et al. Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat Biotechnol 31, 684–686 (2013). https://doi.org/10.1038/nbt.2652

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