References
Zhang, F. et al. Proc. Natl. Acad. Sci. USA 107, 12028–12033 (2010).
Chen, K. & Gao, C. J. Genet. Genomics 40, 271–279 (2013).
Mussolino, C. & Cathomen, T. Nat. Biotechnol. 31, 208–209 (2013).
Barrangou, R. et al. Science 315, 1709–1712 (2007).
Jinek, M. et al. Science 337, 816–821 (2012).
Gasiunas, G., Barrangou, R., Horvath, P. & Siksnys, V. Proc. Natl. Acad. Sci. USA 109, E2579–E2586 (2012).
Cong, L. et al. Science 339, 819–823 (2013).
Jiang, W., Bikard, D., Cox, D., Zhang, F. & Marraffini, L.A. Nat. Biotechnol. 31, 233–239 (2013).
Hwang, W.Y. et al. Nat. Biotechnol. 31, 227–229 (2013).
Chang, N. et al. Cell Res. 23, 465–472 (2013).
Wang, H. et al. Cell 153, 910–918 (2013).
Shan, Q. et al. Mol. Plant 6, 1365–1368 (2013).
Fu, Y. et al. Nat. Biotechnol. advance online publication, doi:10.1038/nbt.2623 (23 June 2013).
Acknowledgements
This work was supported by the National Natural Science Foundation of China (201263, 383601 and 31200273), the Ministry of Agriculture of China (2011ZX08002-004 and 2013ZX08010-002) and Chinese Academy of Sciences (KSCX2-EW-N-06, KSCX2-EW-J-6). We thank X. Wang for bioinformatics analysis.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Supplementary information
Supplementary Text and Figures
Supplementary Methods, Supplementary Figures 1–5 and Supplementary Tables 1–7 (PDF 1175 kb)
Rights and permissions
About this article
Cite this article
Shan, Q., Wang, Y., Li, J. et al. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol 31, 686–688 (2013). https://doi.org/10.1038/nbt.2650
Published:
Issue Date:
DOI: https://doi.org/10.1038/nbt.2650
- Springer Nature America, Inc.
This article is cited by
-
New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i
Plant Methods (2024)
-
Precise integration of large DNA sequences in plant genomes using PrimeRoot editors
Nature Biotechnology (2024)
-
A high-efficient protoplast transient system for screening gene editing elements in Salvia miltiorrhiza
Plant Cell Reports (2024)
-
Efficient CRISPR/Cas9-mediated gene editing of the ZjEIN2 gene in Zoysia japonica
Plant Biotechnology Reports (2024)
-
CRISPR/Cas-Mediated Genome Editing for Sugarcane Improvement
Sugar Tech (2024)