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Genome sequencing in clinical microbiology

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High-throughput sequencing is being adopted to characterize microbial pathogens and track their evolution and spread.

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Figure 1: Clinical microbiological laboratory workflow, now and in the future.

References

  1. Loman, N.J. et al. Nat. Rev. Microbiol. 10, 599–606 (2012).

    Article  CAS  Google Scholar 

  2. Bashir, A. et al. Nat. Biotechnol. 30, 701–707 (2012).

    Article  CAS  Google Scholar 

  3. Koren, S. et al. Nat. Biotechnol. 30, 693–700 (2012).

    Article  CAS  Google Scholar 

  4. Harris, S.R. et al. Science 327, 469–474 (2010).

    Article  CAS  Google Scholar 

  5. Hendriksen, R.S. et al. mBio 2, e00157–11 (2011).

    Article  Google Scholar 

  6. Mutreja, A. et al. Nature 477, 462–465 (2011).

    Article  CAS  Google Scholar 

  7. Rasko, D.A. et al. N. Engl. J. Med. 365, 709–717 (2011).

    Article  CAS  Google Scholar 

  8. Rohde, H. et al. N. Engl. J. Med. 365, 718–724 (2011).

    Article  CAS  Google Scholar 

  9. Chin, C.-S. et al. N. Engl. J. Med. 364, 33–42 (2011).

    Article  CAS  Google Scholar 

  10. Baker, S. et al. Open Biol. 1, 110008 (2011).

    Article  Google Scholar 

  11. Gardy, J.L. et al. N. Engl. J. Med. 364, 730–739 (2011).

    Article  CAS  Google Scholar 

  12. McAdam, P.R. et al. Proc. Natl. Acad. Sci. USA 109, 9107–9112 (2012).

    Article  CAS  Google Scholar 

  13. Eyre, D.W. et al. BMJ Open 2, e001124 (2012).

    Article  Google Scholar 

  14. Köser, C.U. et al. N. Engl. J. Med. 366, 2267–2275 (2012).

    Article  Google Scholar 

  15. Lewis, T. et al. J. Hosp. Infect. 75, 37–41 (2010).

    Article  CAS  Google Scholar 

  16. Nadell, C.D., Xavier, J.B. & Foster, K.R. FEMS Microbiol. Rev. 33, 206–224 (2009).

    Article  CAS  Google Scholar 

  17. Rogers, G.B., Carroll, M.P. & Bruce, K.D. J. Med. Microbiol. 58, 1401–1418 (2009).

    Article  CAS  Google Scholar 

  18. Hall-Stoodley, L., Costerton, J.W. & Stoodley, P. Nat. Rev. Microbiol. 2, 95–108 (2004).

    Article  CAS  Google Scholar 

  19. Cho, I. & Blaser, M.J. Nat. Rev. Genet. 13, 260–270 (2012).

    Article  CAS  Google Scholar 

  20. Rogers, G.B. et al. Gut Microbes 1, 85–93 (2010).

    Article  Google Scholar 

  21. Stecher, B. & Hardt, W.-D. Trends Microbiol. 16, 107–114 (2008).

    Article  CAS  Google Scholar 

  22. de Sablet, T. et al. Infect. Immun. 77, 783–790 (2009).

    Article  CAS  Google Scholar 

  23. Ubeda, C. et al. J. Clin. Invest. 120, 4332–4341 (2010).

    Article  CAS  Google Scholar 

  24. Sen, C.K. et al. Wound Repair Regen. 17, 763–771 (2009).

    Article  Google Scholar 

  25. Tuttle, M.S. et al. J. Clin. Microbiol. 49, 3812–3819 (2011).

    Article  Google Scholar 

  26. Wolcott, R.D., Gontcharova, V., Sun, Y., Zischakau, A. & Dowd, S.E. J. Wound Care 18, 317–323 (2009).

    Article  CAS  Google Scholar 

  27. Greninger, A.L. et al. PLoS ONE 5, e13381 (2010).

    Article  Google Scholar 

  28. Yang, J. et al. J. Clin. Microbiol. 49, 3463–3469 (2011).

    Article  CAS  Google Scholar 

  29. Lieberman, T.D. et al. Nat. Genet. 43, 1275–1280 (2011).

    Article  CAS  Google Scholar 

  30. Croucher, N.J. et al. Science 331, 430–434 (2011).

    Article  CAS  Google Scholar 

  31. Hornsey, M. et al. J. Antimicrob. Chemother. 66, 1499–1503 (2011).

    Article  CAS  Google Scholar 

  32. Feng, J. et al. Genome Res. 19, 1214–1223 (2009).

    Article  CAS  Google Scholar 

  33. Kos, V.N. et al. mBio 3, e00112–12 (2012).

    Article  CAS  Google Scholar 

  34. Fournier, P.-E. & Raoult, D. Annu. Rev. Microbiol. 65, 169–188 (2011).

    Article  CAS  Google Scholar 

  35. Pallen, M.J., Loman, N.J. & Penn, C.W. Curr. Opin. Microbiol. 13, 625–631 (2010).

    Article  CAS  Google Scholar 

  36. Raoult, D., Fournier, P.E. & Drancourt, M. Nat. Rev. Microbiol. 2, 151–159 (2004).

    Article  CAS  Google Scholar 

  37. The NIH HMP Working Group et al. Genome Res. 19, 2317–2323 (2009).

  38. Li, K., Bihan, M., Yooseph, S. & Methé, B.A. PLoS ONE 7, e32118 (2012).

    Article  CAS  Google Scholar 

  39. Wylie, K.M. et al. PLoS ONE 7, e35294 (2012).

    Article  CAS  Google Scholar 

  40. The Human Microbiome Project Consortium. Nature 486, 207–214 (2012).

  41. Sergeant, M.J., Constantinidou, C., Cogan, T., Penn, C.W. & Pallen, M.J. PLoS ONE 7, e38094 (2012).

    Article  CAS  Google Scholar 

  42. Azhikina, T.L. et al. Biotechniques 48, 139–144 (2010).

    Article  CAS  Google Scholar 

  43. Nocker, A. & Camper, A.K. FEMS Microbiol. Lett. 291, 137–142 (2009).

    Article  CAS  Google Scholar 

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Correspondence to Mark J Pallen.

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Competing interests

M.J.P. was a winner of an Ion Torrent Personal Genome Machine in the European PGM grant program.

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Chan, JM., Pallen, M., Oppenheim, B. et al. Genome sequencing in clinical microbiology. Nat Biotechnol 30, 1068–1071 (2012). https://doi.org/10.1038/nbt.2410

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