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Assessing unintended hybridization-induced biological effects of oligonucleotides

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Figure 1: Assessment of hybridization-based off-target effects starts with a mechanism- and chemistry-dependent in silico screen, which is interpreted and validated on a case-by-case basis using auxiliary information and experiments.

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Author information

Authors and Affiliations

  1. Santaris Pharma A/S, Hørsholm, Denmark

    Morten Lindow

  2. Axolabs GmbH, Kulmbach, Germany

    Hans-Peter Vornlocher

  3. DNA Consulting LLC, Ellensburg, Washington, USA

    Donald Riley

  4. Preclinsight, Reno, Nevada, USA

    Douglas J Kornbrust

  5. Merck & Co., Inc., West Point, Pennsylvania, USA

    Julja Burchard

  6. Pfizer, Cambridge, Massachusetts, USA

    Laurence O Whiteley

  7. RXi, Worcester, Massachusetts, USA

    Joanne Kamens

  8. Quark Pharmaceuticals, Boulder, Colorado, USA

    James D Thompson

  9. Alnylam Therapeutics, Cambridge, Massachusetts, USA

    Saraswathy Nochur

  10. Isis Pharmaceuticals, Carlsbad, California, USA

    Husam Younis & Scott P Henry

  11. Merck & Co., Inc., Palo Alto, California, USA

    Steven Bartz

  12. GlaxoSmithKline R&D, Ware, Hertfordshire, UK

    Joel Parry

  13. Topigen Pharmaceuticals, Pharmaxis Ltd., Montreal, Quebec, Canada

    Nicolay Ferrari

  14. miRagen Therapeutics, Boulder, Colorado, USA

    Arthur A Levin

Authors
  1. Morten Lindow
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  2. Hans-Peter Vornlocher
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  3. Donald Riley
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  4. Douglas J Kornbrust
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  5. Julja Burchard
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  6. Laurence O Whiteley
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  7. Joanne Kamens
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  8. James D Thompson
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  9. Saraswathy Nochur
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  10. Husam Younis
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  11. Steven Bartz
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  12. Joel Parry
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  13. Nicolay Ferrari
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  14. Scott P Henry
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  15. Arthur A Levin
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Corresponding author

Correspondence to Arthur A Levin.

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Competing interests

The authors are employees in, or consultants for, commercial companies actively developing oligonucleotides as drugs. See affiliation section for details.

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Lindow, M., Vornlocher, HP., Riley, D. et al. Assessing unintended hybridization-induced biological effects of oligonucleotides. Nat Biotechnol 30, 920–923 (2012). https://doi.org/10.1038/nbt.2376

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  • DOI: https://doi.org/10.1038/nbt.2376

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