Skip to main content
SpringerLink
Log in

Transcriptome sequencing of single cells with Smart-Seq

  • News & Views
  • Published:

From Nature Biotechnology

View current issue Submit your manuscript

A method for performing RNA-Seq on picograms of mRNA facilitates gene expression profiling of rare cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1: Methods for single-cell transcriptomics.

References

  1. Ramsköld, D. et al. Nat. Biotechnol. 30, 777–782 (2012).

    Article  Google Scholar 

  2. Eberwine, J. et al. Proc. Natl. Acad. Sci. USA 89, 3010–3014 (1992).

    Article  CAS  Google Scholar 

  3. Chiang, M.K. & Melton, D.A. Dev. Cell 4, 383–393 (2003).

    Article  CAS  Google Scholar 

  4. Trimarchi, J.M., Stadler, M.B. & Cepko, C.L. PloS One 3, e1588 (2008).

    Article  Google Scholar 

  5. Tietjen, I. et al. Neuron 38, 161–175 (2003).

    Article  CAS  Google Scholar 

  6. Ramos, C.A. et al. PLoS Genet. 2, e159 (2006).

    Article  Google Scholar 

  7. Dalerba, P. et al. Nat. Biotechnol. 29, 1120–1127 (2011).

    Article  CAS  Google Scholar 

  8. Wang, Z., Gerstein, M. & Snyder, M. Nat. Rev. Genet. 10, 57–63 (2009).

    Article  CAS  Google Scholar 

  9. Islam, S. et al. Genome Res. 21, 1160–1167 (2011).

    Article  CAS  Google Scholar 

  10. Tang, F. et al. Nat. Methods 6, 377–382 (2009).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Jillian J. Goetz and Jeffrey M. Trimarchi are in the Department of Genetics, Development and Cell Biology, Neuroscience Program, Iowa State University, Ames, Iowa.,

    Jillian J Goetz & Jeffrey M Trimarchi

Authors
  1. Jillian J Goetz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeffrey M Trimarchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jeffrey M Trimarchi.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goetz, J., Trimarchi, J. Transcriptome sequencing of single cells with Smart-Seq. Nat Biotechnol 30, 763–765 (2012). https://doi.org/10.1038/nbt.2325

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt.2325

  • Springer Nature America, Inc.

This article is cited by

Search

Navigation